Anti-FOXA1 antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab5089
• Prices: $400.00
• Sizes: 100 µg
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P ChIP WB
• Species Reactivities: Mouse, Human, Rat, Bovine, Pig, Xenopus, Zebrafish
• Antigen: Synthetic peptide: C-GVYSRPVLNTS , corresponding to amino acids 463-473 of Human FOXA1(Peptide available as ab23074
• Description: Goat Polyclonal
• Gene: FOXA1
• Conjugate: Unconjugated

Product images

Goat polyclonal to FOXA1 antibody (ab5089) was used in a ChIP assay to detect in vivo binding of FOXA1/HNF3a to the promoter of the TFF1 gene in MCF-7 cells. The cells were fixed in 1% formaldehyde for 15 minutes at room temperature and ChIP was performed using 6µg/ml ab5089. Real-time PCR was used to quantify the enrichment of the TFF1 promoter in the FOXA1 ChIP'd DNA, using the input DNA as the reference. The results indicate that there is an 18-fold enrichment of the TFF1 promoter in the DNA ChIp'd with ab5089.  This image was submitted as part of a review.See Abreview

Chromatin was prepared from nuclear lysate of the human MCF7 breast epithelial adenocarcinoma cells. The cross-linking (X-ChiP) technique was used, crosslinking was performed for 15 minutes in formaldehyde. The primary antibody was diluted to 0.2 μg/μg chromatin and incubated with the sample for 16 hours at 4°C in a dilution buffer that contains SDS, DOC, Triton X-100, EDTA, HEPES, NaCl. The immunoprecipitated DNA was quantified by real time PCR. Ct values were converted to DNA copy numbers using a standard curve in the Q-PCR step. The number of binding events detected for each test reaction was then calculated by taking into account the DNA copy number, cell equivalents of chromatin used in the ChIP and PCR, and primer pair amplification efficiency.See Abreview

ab5089 at 5 µg/ml staining FOXA1 in Human prostate tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product References

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