Anti-FOXC1 antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab5079
• Prices: $400.00
• Sizes: 100 µg
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ChIP WB IHC-P
• Species Reactivities: Mouse, Human, Zebrafish, Xenopus, Fish
• Antigen: Synthetic peptide: RTSGAFVYDCSKF , corresponding to amino acids 541-553 of Human FOXC 1
• Description: Goat Polyclonal
• Gene: FOXC1
• Conjugate: Unconjugated

Product images

Mouse tissue sections (periocular mesenchyme, cornea) were incubated with ab5079, anti-FOXC1 antibody. Paraformaldehyde was used for fixation, and a heat mediated antigen retrieval step was used. The antibody was incubated for 8 hours at a dilution of 1/200.

ab5079 at 3ug/ml staining FOXC1 in human kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in microwave with Tris/EDTA buffer (pH 9.0). The HRP-staining procedure was used for detection.

Lane 1 : Anti-FOXC1 antibody - ChIP Grade (ab5079) at 1 µg/mlLane 2 : Anti-FOXC1 antibody - ChIP Grade (ab5079) at 1 mg/mlLane 3 : Anti-DYKDDDDK tag antibody at 1/3000 dilutionLane 1 : FOXC1-transfected HEK293 lysate with DYKDDDDK tag in RIPA bufferLane 2 : MOCK-transfected HEK293 lysate in RIPA bufferLane 3 : FOXC1-transfected HEK293 lysate with DYKDDDDK tag in RIPA bufferLysates/proteins at 10 µg per lane.developed using the ECL technique

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, staining FOXC1 with ab5079 at 4 µg/ml. Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6).

Product References

Foxc1 is a critical regulator of haematopoietic stem/progenitor cell niche - Foxc1 is a critical regulator of haematopoietic stem/progenitor cell niche

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Foxc1 controls the growth of the murine frontal bone rudiment by direct - Foxc1 controls the growth of the murine frontal bone rudiment by direct

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An unbiased in vivo screen reveals multiple transcription factors that control - An unbiased in vivo screen reveals multiple transcription factors that control

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Initiation of early osteoblast differentiation events through the direct - Initiation of early osteoblast differentiation events through the direct

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A complex regulatory network of transcription factors critical for ocular - A complex regulatory network of transcription factors critical for ocular

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Genetic variations strongly influence phenotypic outcome in the mouse retina. - Genetic variations strongly influence phenotypic outcome in the mouse retina.

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Insulin acts through FOXO3a to activate transcription of plasminogen activator - Insulin acts through FOXO3a to activate transcription of plasminogen activator

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FOXC1 is required for cell viability and resistance to oxidative stress in the - FOXC1 is required for cell viability and resistance to oxidative stress in the

Berry FB, Skarie JM, Mirzayans F, Fortin Y, Hudson TJ, Raymond V, Link BA, Walter MA. Hum Mol Genet. 2008 Feb 15;17(4):490-505. Epub 2007 Nov 9.

Foxc transcription factors directly regulate Dll4 and Hey2 expression by - Foxc transcription factors directly regulate Dll4 and Hey2 expression by

Hayashi H, Kume T. PLoS One. 2008 Jun 11;3(6):e2401.

FGF19 is a target for FOXC1 regulation in ciliary body-derived cells. - FGF19 is a target for FOXC1 regulation in ciliary body-derived cells.

Tamimi Y, Skarie JM, Footz T, Berry FB, Link BA, Walter MA. Hum Mol Genet. 2006 Nov 1;15(21):3229-40. Epub 2006 Sep 25.