Anti-FOXO3A (phospho S253) antibody
| Name | Anti-FOXO3A (phospho S253) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab47285 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-F IHC-P ELISA ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthesized phosphopeptide derived from human FOXO3A around the phosphorylation site of serine 253 (A-V-SP-M-D) |
| Description | Rabbit Polyclonal |
| Gene | FOXO3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-FOXO3A (phospho S253) antibody (ab47285) at 1/500 dilutionLane 1 : extracts from NIH/3T3 cells untreatedLane 2 : extracts from NIH/3T3 cells treated with serumLysates/proteins at 30 µg per lane.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47285 at a 1/50 dilution. Left image : Untreated Right image : Treated with P-Peptide
ICC/IF image of ab47285 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47285, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab47285 at 1/200 dilution staining FOXO3A in human 293FT cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde, permebilized in 0.5% Triton X-100 and then blocked in 5% BSA for 1 hour at 25°C. The primary antibody was used at 1/200 dilution in PBS and incubated with sample at 4°C for 12 hours. An Alexa Fluor® 488 conjugated Goat polyclonal to rabbit IgG was used as secondary at 1/500 dilution. Image show green staining with ab47285 and blue with DAPI.See Abreview
Product References
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Fiskus W, Verstovsek S, Manshouri T, Smith JE, Peth K, Abhyankar S, McGuirk J, Bhalla KN. Mol Cancer Ther. 2013 May;12(5):577-88.
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Fiskus W, Verstovsek S, Manshouri T, Rao R, Balusu R, Venkannagari S, Rao NN, Ha K, Smith JE, Hembruff SL, Abhyankar S, McGuirk J, Bhalla KN. Clin Cancer Res. 2011 Dec 1;17(23):7347-58.
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Jones A, Hwang DJ, Narayanan R, Miller DD, Dalton JT. Endocrinology. 2010 Aug;151(8):3706-19.
Leptin administration favors muscle mass accretion by decreasing FoxO3a and - Leptin administration favors muscle mass accretion by decreasing FoxO3a and
Sainz N, Rodriguez A, Catalan V, Becerril S, Ramirez B, Gomez-Ambrosi J, Fruhbeck G. PLoS One. 2009 Sep 4;4(9):e6808.