FOXO3

Gene Symbol FOXO3
Entrez Gene 2309
Alt Symbol AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A
Species Human
Gene Type protein-coding
Description forkhead box O3
Other Description forkhead box O3A|forkhead box protein O3|forkhead homolog (rhabdomyosarcoma) like 1|forkhead in rhabdomyosarcoma-like 1|forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
Swissprots Q9BZ04 O43524 Q5T2I7 E1P5E6 O15171 B4DVZ6
Accessions CAA04861 EAW48373 EAW48374 O43524 AB072905 AF032886 AAC39592 AI554317 AJ001589 CAA04860 AK024103 AK092357 AK122861 AK301304 BAG62858 AK303933 BAG64861 AK311587 BC020227 AAH20227 BC021224 AAH21224 BC045800 BC068552 AAH68552 CA389775 CD101760 CR749261 CAH18117 XM_005266867 XP_005266924 XM_005266868 XP_005266925 XM_011535626 XP_011533928 XM_011535627 XP_011533929 XM_011535628 XP_011533930 XM_011535629 XP_011533931 NM_001455 NP_001446 NM_201559 NP_963853
Function Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post- transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation. {ECO:0000269|PubMed:10102273, ECO:0000269|PubMed:16751106, ECO:0000269|PubMed:21329882}.
Subcellular Location Cytoplasm, cytosol. Nucleus. Note=Translocates to the nucleus upon oxidative stress and in the absence of survival factors.
Tissue Specificity Ubiquitous. {ECO:0000269|PubMed:9479491}.
Top Pathways Non-small cell lung cancer, Endometrial cancer, Neurotrophin signaling pathway, Chemokine signaling pathway, PI3K-Akt signaling pathway

FoxO3a (75D8) Rabbit mAb (Biotinylated) - 3938 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 75D8
Western blot analysis of extracts from SH-SY5 and HeLa cells using FoxO3a (75D8) Rabbit mAb (Biotinylated).

FoxO3a (D19A7) Rabbit mAb (PE Conjugate) - 14592 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D19A7
Flow cytometric analysis of MRK-nu-1 (blue) and MCF-7 cells (green) using FoxO3a (D19A7) Rabbit mAb (PE Conjugate).

Phospho-FoxO3a (Ser253) (D18H8) Rabbit mAb - 13129 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D18H8
Western blot analysis of extracts from 293T cells, untreated (-) or treated with LY294002 #9901 (50 μM, overnight; +) and Wortmannin #9951 (1 μM, overnight; +), using Phospho-FoxO3a (Ser253) (D18H8) Rabbit mAb (upper) or FoxO3a (D19A7) Rabbit mAb #12829 (lower).

Phospho-FoxO3a (Ser253) Antibody - 9466 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from PC3 cells, untreated or treated with lambda phosphatase, using Phospho-Fox03a (Ser253) Antibody (upper) or Akt Antibody #9272 (lower).

Phospho-FoxO3a (Ser294) Antibody - 5538 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293T cells, transfected with tagged FoxO3a, using Phospho-FoxO3a (Ser294) Antibody.

Phospho-FoxO3a (Ser318/321) Antibody - 9465 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from serum starved and serum-treated NIH/3T3 cells as well as untreated and LY294002/Wortmannin-treated MDA-MB-468 cells, using Phospho-Fox03a (Ser318/321) Antibody (upper) or Akt Antibody #9272 (lower).

Phospho-FoxO3a (Ser413) (D77C9) Rabbit mAb - 8174 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D77C9
Western blot analysis of extracts from SH-SY5Y cells, untreated, MG132-treated, H 2 O 2 -treated or AICAR-treated, using Phospho-FoxO3 (Ser413) (D77C9) Rabbit mAb (upper) and FoxO3a (75D8) Rabbit mAb #2497 (lower).

FoxO3a (75D8) Rabbit mAb - 2497 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 75D8
Western blot analysis of extracts from Jurkat and PC3 cells using FoxO3a (75D8) Rabbit mAb.

FoxO3a (D19A7) Rabbit mAb - 12829 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D19A7
Western blot analysis of extracts from 293T, MRK-nu-1 and Jurkat cells using FoxO3a (D19A7) Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower).

FoxO3a Antibody - 9467 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

FKHRL1 (C-14) - sc-34894 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
FKHRL1 (C-14): sc-34894. Western blot analysis of FKHRL1 expression in non-transfected: sc-117752 (A) and mouse FKHRL1 transfected: sc-178617 (B) 293T whole cell lysates.

FKHRL1 (C-18) - sc-34895 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
FKHRL1 (C-18): sc-34895. Western blot analysis of FKHRL1 expression in non-transfected: sc-117752 (A) and mouse FKHRL1 transfected: sc-178617 (B) 293T whole cell lysates.

FKHRL1 (N-15) - sc-34897 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
FKHRL1 (N-15): sc-34897. Western blot analysis of FKHRL1 expression in HeLa (A) and NIH/3T3 (B) nuclear extracts.

FKHRL1 (H-144) - sc-11351 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
FKHRL1 (H-144): sc-11351. Western blot analysis of FKHRL1 expression in PDGF-treated NIH/3T3 whole cell lysate.

FKHRL1 (D-12) - sc-48348 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
FKHRL1 (D-12): sc-48348. Western blot analysis of human recombinant FKHRL1.

FKHRL1 (S-20) - sc-9812 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
FKHRL1 (S-20): sc-9812. Western blot analysis of FKHRL1 expression in TE671 whole cell lysate.

FKHRL1 (N-16) - sc-9813 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
FKHRL1 (N-16): sc-9813. Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of glandular cells.

p-FKHRL1 (Thr 32) - sc-12357 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-FKHRL1 (Thr 32): sc-12357. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.

p-FKHRL1 (Ser 253) - sc-12897 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-FKHRL1 (Ser 253)-R: sc-12897-R. Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of glandular cells.

p-FKHRL1 (Ser 253) - sc-101683 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-FKHRL1 (Ser 253): sc-101683. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.

Anti-FOXO3A (phospho S253) antibody - ab47285 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-FOXO3A (phospho S253) antibody (ab47285) at 1/500 dilutionLane 1 : extracts from NIH/3T3 cells untreatedLane 2 : extracts from NIH/3T3 cells treated with serumLysates/proteins at 30 µg per lane.

Anti-FOXO3A (phospho S253) antibody - ab31109 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
FOXO3A was immunoprecipitated using 0.5mg PC12 whole cell extract, 5µg of Rabbit polyclonal to FOXO3A and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, PC12 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31109.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 63kDa; FOXO3A

Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] - ab154786 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1951(2)
All lanes : Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (ab154786) at 1/1000 dilutionLane 1 : MCF-7 cell lysate untreatedLane 2 : MCF-7 cell lysate treated with IGFLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-FOXO3A (phospho T32) antibody - ab26649 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-FOXO3A (phospho T32) antibody (ab26649) at 1 µg/mlLane 1 : HT29 (Human colon adenocarcinoma grade II cell line) Whole Cell Lysate (ab3952)Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : 293T Cell extract - treated with Calyculin ALane 4 : HT29 (Human colon adenocarcinoma grade II cell line) Whole Cell Lysate (ab3952) with Human FOXO3A (phospho T32) peptide (ab27877) at 1 mg/mlLane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human FOXO3A (phospho T32) peptide (ab27877) at 1 mg/mlLane 6 : 293T Cell extract - treated with Calyculin A with Human FOXO3A (phospho T32) peptide (ab27877) at 1 mg/mlLane 7 : HT29 (Human colon adenocarcinoma grade II cell line) Whole Cell Lysate (ab3952) with Human FOXO3A peptide (ab27878) at 1 mg/mlLane 8 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human FOXO3A peptide (ab27878) at 1 mg/mlLane 9 : 293T Cell extract - treated with Calyculin A with H

Anti-FOXO3A antibody - ab47409 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue in the presence (right) and absence (left) of blocking peptide, using ab47409 at 1/50 dilution.
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