Anti-FOXO3A antibody
| Name | Anti-FOXO3A antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab47409 |
| Prices | $359.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IP WB IHC-P ELISA |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | The antiserum was produced against synthesized non-phosphopeptide derived from human FKHRL1 around the phosphorylation site of serine 253 |
| Description | Rabbit Polyclonal |
| Gene | FOXO3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue in the presence (right) and absence (left) of blocking peptide, using ab47409 at 1/50 dilution.
All lanes : Anti-FOXO3A antibody (ab47409) at 1/500 dilutionLane 1 : NIH/3T3 cell extractsLane 2 : NIH/3T3 cell extracts with Blocking peptide
ICC/IF image of ab47409 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47409, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab47409 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47409, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Product References
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FOXO3a inhibits TNF-alpha- and IL-1beta-induced astrocyte - FOXO3a inhibits TNF-alpha- and IL-1beta-induced astrocyte
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