Anti-FOXO4 antibody
| Name | Anti-FOXO4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab63254 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic non-phosphopeptide derived from human FOXO4 around the phosphorylation site of threonine 451 |
| Description | Rabbit Polyclonal |
| Gene | FOXO4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-FOXO4 antibody (ab63254) at 1/500 dilutionLane 1 : extracts from K562 cellsLane 2 : extracts from K562 cells, with immunizing peptide
Immunofluorescence analysis of FOXO4 expression in HeLa cells, using 1/500 ab63254. Left: untreated sample. Right: sample treated with immunising peptide.
Ab63254 staining Human normal pancreas. Staining is localised to the nucleus and cytoplasm.Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.