FOXO4

Gene Symbol FOXO4
Entrez Gene 4303
Alt Symbol AFX, AFX1, MLLT7
Species Human
Gene Type protein-coding
Description forkhead box O4
Other Description fork head domain transcription factor AFX1|forkhead box protein O4|myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7
Swissprots Q8TDK9 O43821 B7WPJ7 P98177 Q13720 Q3KPF1
Accessions CAA72156 EAX05321 EAX05322 P98177 AF384029 AAL85197 AK300142 BAG61928 AW292413 BC026735 BC106761 AAI06762 BP349083 U10072 AAA82171 X93996 CAA63819 XM_011530957 XP_011529259 NM_001170931 NP_001164402 NM_005938 NP_005929
Function Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity. {ECO:0000269|PubMed:10217147, ECO:0000269|PubMed:10783894, ECO:0000269|PubMed:12761217, ECO:0000269|PubMed:15126506, ECO:0000269|PubMed:16054032, ECO:0000269|PubMed:16964248, ECO:0000269|PubMed:20874444, ECO:0000269|PubMed:22972301}.
Subcellular Location Cytoplasm. Nucleus. Note=When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.
Tissue Specificity Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.

Phospho-FoxO4 (Ser193) Antibody - 9471 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Cos cells, serum-starved or with serum treatment, using Phospho-Fox04 (Ser193) Antibody.

FoxO4 Antibody - 9472 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Ramos and Jurkat cells, using Fox04 Antibody.

FoxO4 (55D4) Rabbit mAb - 2499 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 55D4
Western blot analysis of extracts from Jurkat and U937 cells using Fox04 Antibody.

AFX1 (N-19) - sc-5221 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
AFX1 (N-19): sc-5221. Western blot analysis of AFX1 expression in K-562 (A), Jurkat (B) and CCRF-CEM (C) whole cell lysates.

AFX1 (C-16) - sc-5224 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
AFX1 (C-16): sc-5224. Western blot analysis of AFX1 expression in MM-142 (A), LADMAC (B) and C4 (C) whole cell lysates.

AFX1 (H-80) - sc-25359 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
AFX1 (H-80): sc-25359. Western blot analysis of AFX1 expression in HeLa (A), MM-142 (B), LADMAC (C) and c4 (D) whole cell lysates.

AFX1 (N-16) - sc-34902 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

AFX1 (E-17) - sc-34899 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
AFX1 (E-17): sc-34899. Western blot analysis of AFX1 expression in BJAB (A), Ramos (B), Jurkat (C) and Hep G2 (D) nuclear extracts.

p-AFX1 (Ser 197) - sc-101628 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-AFX1 (Ser 197): sc-101628. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.

Anti-FOXO4 (phospho S193) antibody - ab2560 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab2560 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2560, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-FOXO4 (phospho S193) antibody [EPR2150(N)] - ab174849 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2150(N)
All lanes : Anti-FOXO4 (phospho S193) antibody [EPR2150(N)] (ab174849) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : 293T cell lysate, transfected with FOXO4Lane 3 : 293T cell lysate, transfected with FOXO4.A3 (T32A, S197(193)A, S262A)SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-FOXO4 (phospho S197) antibody - ab47278 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffinembedded human breast carcinoma tissue using ab47278. Right : peptide 1ug/ml

Anti-FOXO4 (phospho S197) antibody - ab192183 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-FOXO4 (phospho S197) antibody (ab192183) at 1/500 dilutionLane 1 : Serum treated 293 whole cell lysate with the antigen-specific peptideLane 2 : Serum treated 293 whole cell lysateLysates/proteins at 12.5 µg per lane.

Anti-FOXO4 (phospho S262) antibody - ab79267 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Left panel: immunohistochemistry analysis of paraffin-embedded Human brain tissue using 1/50 ab79267. Right panel: same, after pre-incubation with the immunizing peptide.

Anti-FOXO4 (phospho S262) antibody [EPR2315(2)] - ab126594 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2315(2)
All lanes : Anti-FOXO4 (phospho S262) antibody [EPR2315(2)] (ab126594) at 1/10000 dilutionLane 1 : Cell lysate from FOXO4 transfected 293TLane 2 : Cell lysate from FOXO4 transfected 293T treated with Alkaline PhosphataseLane 3 : Cell lysate from non-transfected 293TLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-FOXO4 (phospho T32) antibody [EPR2314(2)] - ab128876 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2314(2)
All lanes : Anti-FOXO4 (phospho T32) antibody [EPR2314(2)] (ab128876) at 1/1000 dilutionLane 1 : 293T cell lysatesLane 2 : 293T transfected with HA-FOXO4 cell lysatesLane 3 : 293T transfected with HA-FOXO4.A3 (T32, S197 and S262 mutated to A) cell lysates

Anti-FOXO4 (phospho T451) antibody - ab79188 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-FOXO4 (phospho T451) antibody (ab79188) at 1/500 dilutionLane 1 : Extracts from HUVEC cells treated with EGF (200ng/ml, 5mins)Lane 2 : Extracts from HUVEC cells treated with EGF (200ng/ml, 5mins) with immunising phosphopeptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-FOXO4 (phospho T451) antibody - ab195818 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-FOXO4 (phospho T451) antibody (ab195818) at 1/500 dilutionLane 1 : EGF treated Huvec whole cell lysates with antigen-specific peptideLane 2 : EGF treated Huvec whole cell lysatesLysates/proteins at 12.5 µg per lane.

Anti-FOXO4 antibody - ab63254 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-FOXO4 antibody (ab63254) at 1/500 dilutionLane 1 : extracts from K562 cellsLane 2 : extracts from K562 cells, with immunizing peptide

Anti-FOXO4 antibody - ab47396 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-FOXO4 antibody (ab47396) at 1/500 dilutionLane 1 : Extracts from 293 cells treated with serumLane 2 : Extracts from 293 cells treated with serum and peptide

Anti-FOXO4 antibody - ab154520 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-FOXO4 antibody (ab154520) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

Anti-FOXO4 antibody - ab60271 from Abcam

Host Goat
Clonality Polyclonal
Anti-FOXO4 antibody (ab60271) at 1 µg/ml + K562 cell lysate (35µg protein in RIPA buffer)

Anti-FOXO4 antibody - ab24505 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-FOXO4 antibody - ab92893 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-FOXO4 antibody (ab92893) at 1/500 dilutionLane 1 : 3T6 cell lysate at 3 µgLane 2 : 3T6 cell lysate at 10 µgLane 3 : 3T6 cell lysate at 30 µg

Anti-FOXO4 antibody - ab60086 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Ab60086 (1/50-1/100) staining human FOXO4 in human cancer tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. HC = hepatocarcinoma.
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