All lanes : Anti-FOXP1 antibody - C-terminal (ab191184) at 1/1000 dilutionLane 1 : A549 cell line lysateLane 2 : Daudi cell line lysateLane 3 : Jurkat cell line lysateLane 4 : MCF7 cell line lysateLane 5 : NCI-N292 cell line lysateLysates/proteins at 35 µg per lane.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue labeling FOXP1 with ab191184 at 1/75 dilution.
Flow cytometric analysis of Ramos cells labeling FOXP1 with ab191184 at 1/10 dilution (right histogram) compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.