Anti-Fragilis antibody [EPR5242]

• Supplier: Abcam
• Catalog: ab109429
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR5242
• Applications: WB IHC-P ICC/IF
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)
• Description: Rabbit Monoclonal
• Gene: IFITM3
• Conjugate: Unconjugated

Product images

All lanes : Anti-Fragilis antibody [EPR5242] (ab109429) at 1/1000 dilutionLane 1 : HeLa cell lysate Lane 2 : 293T cell lysate Lane 3 : Human placenta lysateLysates/proteins at 10 µg per lane.

Immunohistochemical analysis of Fragilis in paraffin-embedded Human kidney tissue using ab109429 at 1/250 dilution.

Anti-Fragilis antibody [EPR5242] (ab109429) at 1/1000 dilution + Human Fragilis full length protein (ab92921) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 10 seconds

ICC/IF image of ab109429 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109429 at 1/50 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.