Normal human lymphoblast cells were stained with 1 µg/mL ab113691 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
![All lanes : Anti-Frataxin antibody [17A11] (ab113691)Lane 1 : Recombinant frataxin at 0.1 µgLane 2 : Human heart mitochondria at 15 µgLane 3 : Human liver mitochondria at 15 µgLane 4 : HepG2 whole cell lysate at 15 µgLane 5 : Bovine heart mitochondria at 15 µgLane 6 : H9C2 rat whole cell lysate at 15 µgLane 7 : MEF mouse embryonic fibroblast at 15 µgLane 8 : Mouse liver mitochondria at 15 µgLane 9 : Rat liver mitochondria at 15 µg](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/20874_Frataxin-Primary-antibodies-ab113691-8.jpg)
All lanes : Anti-Frataxin antibody [17A11] (ab113691)Lane 1 : Recombinant frataxin at 0.1 µgLane 2 : Human heart mitochondria at 15 µgLane 3 : Human liver mitochondria at 15 µgLane 4 : HepG2 whole cell lysate at 15 µgLane 5 : Bovine heart mitochondria at 15 µgLane 6 : H9C2 rat whole cell lysate at 15 µgLane 7 : MEF mouse embryonic fibroblast at 15 µgLane 8 : Mouse liver mitochondria at 15 µgLane 9 : Rat liver mitochondria at 15 µg
ab113691 stained fibroblast cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (ab113691, 1µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix.
IHC image of Frataxin staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113691, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.