All lanes : Anti-FRG1 antibody [EPR13098] (ab181083) at 1/10000 dilutionLane 1 : HeLa whole cell lysateLane 2 : Jurkat whole cell lysateLane 3 : 293 whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescent analysis of HeLa cells labeling FRG1 with ab181083 at a 1/100 dilution. Primary incubation was followed by secondary antibody labeling with Alexa Fluor 555-conjugated goat anti-rabbit lgG (red - left panel). DAPI was used to stain the cell nuclear (blue - right panel).
Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate labeling FRG1 with ab181083 at 1/30 dilution. Right lane is the negative control.