Anti-Frizzled 5 antibody
| Name | Anti-Frizzled 5 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab75234 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Human, Rat |
| Antigen | Synthetic peptide derived from an internal region of human Frizzled 5 |
| Description | Rabbit Polyclonal |
| Gene | FZD5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab75234 at 1/500 dilution staining Frizzled 5 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
All lanes : Anti-Frizzled 5 antibody (ab75234) at 1/500 dilutionLane 1 : LOVO cell extractsLane 2 : LOVO cell extracts with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.
ab75234 (4µg/ml) staining Frizzled 5 in human pancreas using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of the Islets of Langerhans.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Product References
Bone marrow mesenchymal stem cell-derived Wnt5a inhibits leukemia cell - Bone marrow mesenchymal stem cell-derived Wnt5a inhibits leukemia cell
Shen YL, Luo Q, Guo YX, Zheng GH, Yu J, Xu YH. Oncol Lett. 2014 Jul;8(1):85-90. Epub 2014 May 7.
Monocyte-secreted Wnt5a interacts with FZD5 in microvascular endothelial cells - Monocyte-secreted Wnt5a interacts with FZD5 in microvascular endothelial cells
Arderiu G, Espinosa S, Pena E, Aledo R, Badimon L. J Mol Cell Biol. 2014 Oct;6(5):380-93.
Spatio-temporal expression pattern of frizzled receptors after contusive spinal - Spatio-temporal expression pattern of frizzled receptors after contusive spinal
Gonzalez P, Fernandez-Martos CM, Gonzalez-Fernandez C, Arenas E, Rodriguez FJ. PLoS One. 2012;7(12):e50793.
Paneth cell marker expression in intestinal villi and colon crypts characterizes - Paneth cell marker expression in intestinal villi and colon crypts characterizes
Wang D, Peregrina K, Dhima E, Lin EY, Mariadason JM, Augenlicht LH. Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10272-7. doi:
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