Anti-FTO antibody - N-terminal (ab135628) at 1/100 dilution + NCI-H460 cells lysate at 35 µg
Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human brain tissue labelling FTO with ab135628 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
Flow cytometric analysis of NCI-H292 cells using ab135628 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC conjugated goat anti-rabbit secondary antibodies were used for the analysis.