FTO

Gene Symbol FTO
Entrez Gene 79068
Alt Symbol ALKBH9
Species Human
Gene Type protein-coding
Description fat mass and obesity associated
Other Description AlkB homolog 9|alpha-ketoglutarate-dependent dioxygenase FTO|fat mass and obesity-associated protein|protein fto
Swissprots Q9C0B1 B2RNS0 A2RUH1 Q7Z785 Q0P676
Accessions EAW82811 Q9C0B1 AB051539 BAB21843 AK096554 BAG53322 AK295196 BAG58196 BC001284 BC003583 AAH03583 BC030798 AAH30798 BC132892 AAI32893 BC137091 AAI37092 BC148442 AAI48443 BC153032 AAI53033 DA324897 U79260 AAB50206 XM_011523313 XP_011521615 XM_011523314 XP_011521616 XM_011523315 XP_011521617 XM_011523316 XP_011521618 NM_001080432 NP_001073901
Function Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single- stranded RNA containing 3-methyluracil, followed by single- stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Has no activity towards 1- methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation. {ECO:0000269|PubMed:18775698, ECO:0000269|PubMed:20376003, ECO:0000269|PubMed:22002720}.
Subcellular Location Nucleus {ECO:0000269|PubMed:22002720}. Nucleus speckle {ECO:0000269|PubMed:22002720}.
Tissue Specificity Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary. {ECO:0000269|PubMed:17434869, ECO:0000269|PubMed:17496892}.

FTO Antibody - 14386 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from SH-SY5Y and HeLa cells using FTO Antibody.

Fatso (C-3) - sc-271713 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Fatso (C-3): sc-271713. Western blot analysis of Fatso expression in SK-N-MC (A) and SW-13 (B) whole cell lysates.

Fatso (L-12) - sc-83811 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Fatso (L-12): sc-83811. Western blot analysis of Fatso expression in rat brain tissue extract.

Fatso (H-300) - sc-98768 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Fatso (H-300): sc-98768. Western blot analysis of Fatso expression in mouse pancreas tissue extract (A) and MIA PaCa-2 whole cell lysate (B).

Fatso (W-13) - sc-83813 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-FTO antibody - ab94482 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-FTO antibody (ab94482) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Mouse Hippocampus Tissue LysateLane 3 : Brain (Mouse) Tissue LysateLane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-FTO antibody - ab106386 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-FTO antibody (ab106386) at 1 µg/mlLane 2 : Anti-FTO antibody (ab106386) at 2 µg/mlLane 1 : Human uterus tissue lysate Lane 2 : Human uterus tissue lysate Lysates/proteins at 15 µg per lane.

Anti-FTO antibody - ab77547 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-FTO antibody (ab77547) at 0.03 µg/ml + Human Pancreas lysate (in RIPA buffer) at 35 µgdeveloped using the ECL technique

Anti-FTO antibody [5-2H10] - ab92821 from Abcam

Prices $385.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5-2H10
Anti-FTO antibody [5-2H10] (ab92821) at 1/1000 dilution + Rat testes lysate

Anti-FTO antibody [EPR6894] - ab126605 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6894
Immunohistochemical staining of paraffin embedded human hepatocellular carcinoma with purified ab126605 at a working dilution of 1 in 500. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Anti-FTO antibody [EPR6895] - ab124892 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6895
All lanes : Anti-FTO antibody [EPR6895] (ab124892) at 1/1000 dilutionLane 1 : 293T lysateLane 2 : SH-SY5Y lysateLane 3 : Caco2 lysateLane 4 : BxPC3 lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-FTO antibody - N-terminal - ab135628 from Abcam

Prices $384.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-FTO antibody - N-terminal (ab135628) at 1/100 dilution + NCI-H460 cells lysate at 35 µg

Anti-FTO Antibody (C-Terminus) - LS-C129179 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-FTO Antibody (C-Terminus) - LS-C129180 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-FTO Antibody (C-Terminus, Biotin) - LS-C141700 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-FTO Antibody (C-Terminus, DY650) - LS-C141701 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-FTO Antibody (C-Terminus, DY488) - LS-C141702 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-FTO Antibody (C-Terminus, HRP) - LS-C141703 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-FTO Antibody (C-Terminus, DY550) - LS-C141704 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-FTO Antibody (C-Terminus) - LS-C187751 from LifeSpan Bioscience

Prices $360.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-FTO Antibody (N-Terminus, AP) - LS-C244434 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-FTO Antibody (N-Terminus) - LS-C108502 from LifeSpan Bioscience

Prices $345.00, $1,035.00
Sizes 100 µg, 500 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence of FTO in Mouse Brain cells with FTO antibody at 20 ug/ml.

Anti-FTO Antibody (N-Terminus, FITC) - LS-C244436 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-FTO Antibody (N-Terminus, APC) - LS-C244435 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-FTO Antibody (N-Terminus, PE) - LS-C244438 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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