Anti-Furin antibody
| Name | Anti-Furin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab3467 |
| Prices | $398.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF B/N ICC/IF ICC/IF IP WB IHC-P |
| Species Reactivities | Mouse, Rat, Sheep, Hamster, Dog, Human, Pig, Primate |
| Antigen | Synthetic peptide corresponding to Human Furin aa 780-793 |
| Description | Rabbit Polyclonal |
| Gene | FURIN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunolocalization of endogenous furin in mouse 3T3 cells with ab3467.
All lanes : Anti-Furin antibody (ab3467) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab3467 staining Furin in human A549 cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed with paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 1% BSA/ 5%FBS/PBS for 1 hour at 25°C and then incubated with ab3467 at a 1/100 dilution for 1 hour at 25°C. The secondary used was an Alexa-Fluor 546 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.Cells were counterstained with DAPI (436nM, 5 mins) and Phalloidin-633 (1unit/coverslip) and mounted with prolong gold. No staining seen in secondary control and furin staining was punctate and throughout the cytoplasm.See Abreview
Product References
Proprotein convertase furin regulates apoptosis and proliferation of granulosa - Proprotein convertase furin regulates apoptosis and proliferation of granulosa
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Relevance of vascular peroxisome proliferator-activated receptor gamma - Relevance of vascular peroxisome proliferator-activated receptor gamma
Valero-Munoz M, Martin-Fernandez B, Ballesteros S, Martinez-Martinez E, Blanco-Rivero J, Balfagon G, Cachofeiro V, Lahera V, de las Heras N. Exp Physiol. 2013 May;98(5):999-1008.
SORLA-dependent and -independent functions for PACS1 in control of amyloidogenic - SORLA-dependent and -independent functions for PACS1 in control of amyloidogenic
Burgert T, Schmidt V, Caglayan S, Lin F, Fuchtbauer A, Fuchtbauer EM, Nykjaer A, Carlo AS, Willnow TE. Mol Cell Biol. 2013 Nov;33(21):4308-20.
Regulation of PKD by the MAPK p38delta in insulin secretion and glucose - Regulation of PKD by the MAPK p38delta in insulin secretion and glucose
Sumara G, Formentini I, Collins S, Sumara I, Windak R, Bodenmiller B, Ramracheya R, Caille D, Jiang H, Platt KA, Meda P, Aebersold R, Rorsman P, Ricci R. Cell. 2009 Jan 23;136(2):235-48.
Effect of ultraviolet radiation on the expression of pp38MAPK and furin in human - Effect of ultraviolet radiation on the expression of pp38MAPK and furin in human
Huynh TT, Chan KS, Piva TJ. Photodermatol Photoimmunol Photomed. 2009 Feb;25(1):20-9. doi:
The Ca2+-binding capacity of epidermal furin is disrupted by H2O2-mediated - The Ca2+-binding capacity of epidermal furin is disrupted by H2O2-mediated
Spencer JD, Gibbons NC, Bohm M, Schallreuter KU. Endocrinology. 2008 Apr;149(4):1638-45.
Dual mechanisms for the fibrate-mediated repression of proprotein convertase - Dual mechanisms for the fibrate-mediated repression of proprotein convertase
Kourimate S, Le May C, Langhi C, Jarnoux AL, Ouguerram K, Zair Y, Nguyen P, Krempf M, Cariou B, Costet P. J Biol Chem. 2008 Apr 11;283(15):9666-73.
Prohormone convertases 1/3, 2, furin and protein 7B2 (Secretogranin V) in - Prohormone convertases 1/3, 2, furin and protein 7B2 (Secretogranin V) in
Portela-Gomes GM, Grimelius L, Stridsberg M. Regul Pept. 2008 Feb 7;146(1-3):117-24. Epub 2007 Sep 19.