All lanes : Anti-FXYD1 [EPR16081] antibody (ab191397) at 1/10000 dilutionLane 1 : Human skeletal muscle lysateLane 2 : Human fetal heart lysateLane 3 : Human fetal liver lysateLysates/proteins at 20 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling FXYD1 with ab191397 at 1/700 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.Inset: Negative control: using PBS instead of primary antibody.
Western blot analysis of immunoprecipitation pellet from Human fetal livert lysate immunoprecipitated using ab191397 at 1/90 dilution.Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.