Anti-FXYD1 antibody [EPR16082]

• Supplier: Abcam
• Catalog: ab200204
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16082
• Applications: WB IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human FXYD1 aa 50 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: FXYD1
• Conjugate: Unconjugated

Product images

Anti-FXYD1 antibody [EPR16082] (ab200204) at 1/1000 dilution + Human skeletal muscle lysate at 20 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-FXYD1 antibody [EPR16082] (ab200204) at 1/10000 dilutionLane 1 : Human fetal heart lysateLane 2 : Human fetal liver lysateLysates/proteins at 20 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-FXYD1 antibody [EPR16082] (ab200204) at 1/1000 dilutionLane 1 : Mouse heart lysateLane 2 : Rat heart lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

FXYD1 was immunoprecipitated from 1mg of Human fetal heart whole cell lysate with ab200204 at 1/120 dilution. Western blot was performed using ab200204 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: Human fetal heart whole cell lysate 10ug (Input).Lane 2: ab200204 IP in Human fetal heart whole cell lysate.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200204 in Human fetal heart whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.