Anti-Fyn (phospho Y530) + Yes (phospho Y537) antibody [EPR13512]

• Supplier: Abcam
• Catalog: ab188319
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR13512
• Applications: FC WB
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Fyn (phospho Y530) + Yes (phospho Y537) aa 500 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: FYN
• Conjugate: Unconjugated

Product images

All lanes : Anti-Fyn (phospho Y530) + Yes (phospho Y537) antibody [EPR13512] (ab188319) at 1/20000 dilutionLane 1 : Lysate from HeLa cells treated with pervanadateLane 2 : Lysate from untreated HeLa cellsLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Flow cytometric analysis of 2% paraformaldehyde-fixed, permeabilized HeLa cells, untreated (green) or pervanadate-treated (red) labeling Fyn (phospho Y530) + Yes (phospho Y537) with ab188319 at 1/80 dilution, and pervanadate-treated HeLa cells using ab188319 pre-incubated with phospho-Fyn (phospho Y530) + Yes (phospho Y537) peptide (blue) or non-phospho-Fyn (phospho Y530) + Yes (phospho Y537) peptide (orange). Goat anti rabbit IgG (FITC) secondary antibody was used at 1/150 dilution.