FYN

Gene Symbol FYN
Entrez Gene 2534
Alt Symbol SLK, SYN, p59-FYN
Species Human
Gene Type protein-coding
Description FYN proto-oncogene, Src family tyrosine kinase
Other Description FYN oncogene related to SRC, FGR, YES|OKT3-induced calcium influx regulator|c-syn protooncogene|proto-oncogene Syn|proto-oncogene c-Fyn|src-like kinase|src/yes-related novel|tyrosine kinase p59fyn(T)|tyrosine-protein kinase Fyn
Swissprots H0UI48 E1P557 Q5R3A7 Q5R3A6 Q16248 B5BU57 P06241 Q8N5D7
Accessions AAA52491 AAA52492 EAW48278 EAW48279 EAW48280 EAW48281 EAW48282 EAW48283 EAW48284 P06241 AB451293 BAG70107 AB451426 BAG70240 AI187118 AK056699 BAG51788 AY429536 BC032496 AAH32496 BI552534 BP391373 CX781584 CX872011 DA570412 DA815564 DA922886 DA931034 JF432194 ADZ15411 M14333 AAC08285 M14676 AAA36615 S74774 AAB33113 XM_005266890 XP_005266947 XM_005266892 XP_005266949 XM_011535662 XP_011533964 XM_011535663 XP_011533965 XM_011535664 XP_011533966 XM_011535665 XP_011533967 XM_011535666 XP_011533968 XM_011535667 XP_011533969 XM_011535668 XP_011533970 NM_002037 NP_002028 NM_153047 NP_694592 NM_153048 NP_694593 NM_001242779
Function Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta- catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an esse
Subcellular Location Cytoplasm. Nucleus. Cell membrane. Note=Present and active in lipid rafts. Palmitoylation is crucial for proper trafficking.
Tissue Specificity Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T-lymphocytes. {ECO:0000269|PubMed:10196263}.
Top Pathways Sphingolipid signaling pathway, Measles, Adherens junction, Osteoclast differentiation, Prion diseases

Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb - 6943 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D49G4
Western blot analysis of extracts NIH/3T3 cells, serum-starved or treated with human Platelet-Derived Growth Factor BB hPDGF-BB #8912 (100 ng/ml, 15 min), using Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb (upper) or Src (36D10) Rabbit mAb #2109 (lower).

Phospho-Src Family (Tyr416) Antibody - 2101 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from serum-starved COLO 201 cells, v-Src antibody immunoprecipitates from COLO 201 cells and PDGF-treated NIH/3T3 cells, treated with lambda phosphatase as indicated, using Phospho-Src (Tyr416) Antibody (upper) or v-Src antibody (lower).

Fyn Antibody - 4023 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of cell extracts from various cell lines, using Fyn Antibody.

Non-phospho-Src (Tyr416) (7G9) Mouse mAb - 2102 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 7G9
Western blot analysis of extracts from pervanadate-treated (1 mM for 5 minutes) NIH/3T3 cells that have been stably transfected with a constitutively active form of Src (in which the regulatory tyrosine 527 residue has been mutated to phenylalanine), A431 cells and HepG2 cells, using Non-phospho-Src (Tyr416) (7G9 ) Mouse mAb (top), v-Src antibody (middle) or Phospho-Src (Tyr416) Antibody #2101 (bottom).

Phospho-Src Family (Tyr416) (100F9) Rabbit mAb - 2113 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone 100F9

c-Src (B-12) - sc-8056 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
c-Src (B-12): sc-8056. Western blot analysis of human recombinant c-Src.

p-c-Src (pY530.31) - sc-136012 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
p-c-Src (pY530.31): sc-136012. Western blot analysis of c-Src phosphorylation in EGF-treated A-431 whole cell lysates either untreated (A) or treated (B) with alkaline phosphatase.

Fyn (E-3) - sc-365913 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Fyn (E-3): sc-365913. Western blot analysis of Fyn expression in non-transfected 293T: sc-117752 (A), human Fyn transfected 293T: sc-115186 (B) and HeLa (C) whole cell lysates.

Fyn (E-12) - sc-271294 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Fyn (E-12): sc-271294. Western blot analysis of Fyn expression in HeLa (A) and NIH/3T3 (B) whole cell lysates.

Fyn (FYN3) - sc-16 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of NIH/3T3 cells (A,C,E) and NIH/3T3 cells transfected with a Fyn expression vector (B,D,F) using Fyn (FYN3): sc-16 (A,B), Fyn (FYN3)-G: sc-16-G (C,D) and Fyn (15): sc-434 (E,F).

Fyn (G-4) - sc-271293 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Fyn (G-4): sc-271293. Western blot analysis of Fyn expression in HeLa (A) and NIH/3T3 (B) whole cell lysates.

Fyn (H-12) - sc-30680 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Fyn (H-80) - sc-28791 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Fyn (H-80): sc-28791. Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.

Fyn (N-18) - sc-30678 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Fyn (15) - sc-434 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Fyn (15) PE: sc-434 PE. Intracellular FCM analysis of fixed and permeabilized human peripheral blood leukocytes. Black line histogram represents the isotype control, normal mouse IgG<sub>1</sub>: sc-2866.

Fyn (FYN-01) - sc-51598 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Fyn (FYN-01): sc-51598. Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and nuclear localization..

Fyn (6D406) - sc-71133 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Fyn (6D406): sc-71133. Western blot analysis of Fyn expression in HuT 78 (A), Jurkat (B), HeLa (C) and M1 (D) whole cell lysates.

Fyn (FYN-59) - sc-73388 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/500 µl
Host Mouse
Clonality Monoclonal
Fyn (FYN-59): sc-73388. Western blot analysis of Fyn expression in HuT 78 (A), Jurkat (B), HeLa (C) and M1 (D) whole cell lysates.

p-Fyn (Thr 12) - sc-16848 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-Fyn (Thr 12)-R: sc-16848-R. Western blot analysis of Fyn phosphorylation in untreated (A) and lambda protein phosphatase treated (B) mouse brain tissue extracts.

p-Fyn (D-1) - sc-377555 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of Fyn phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) mouse brain tissue extracts. Antibodies tested include p-Fyn (D-1): sc-377555 (A,B) and Fyn (H-80): sc-28791 (C,D).

Anti-Fyn (phospho Y530) + Yes (phospho Y537) antibody [EPR13512] - ab188319 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13512
All lanes : Anti-Fyn (phospho Y530) + Yes (phospho Y537) antibody [EPR13512] (ab188319) at 1/20000 dilutionLane 1 : Lysate from HeLa cells treated with pervanadateLane 2 : Lysate from untreated HeLa cellsLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Anti-Fyn (phospho Y530) antibody - ab182661 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Fyn (phospho Y530) antibody (ab182661) at 1/500 dilutionLane 1 : Mouse brain tissue extract with antigen-specific peptideLane 2 : Mouse brain tissue extract

Anti-Fyn (phospho Y530) antibody - ab192172 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Fyn (phospho Y530) antibody (ab192172) at 1/500 dilutionLane 1 : H2O2 treated 293 whole cell lysates treated with the antigen-specific peptideLane 2 : H2O2 treated 293 whole cell lysatesLysates/proteins at 12.5 µg per lane.

Anti-Fyn antibody - ab13955 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab13955 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13955, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Fyn antibody - ab71676 from Abcam

Prices $379.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-Fyn antibody (ab71676) at 1/100 dilution + mouse liver tissue lysate at 12.5 µgSecondarySecondary HRP-anti-rabbit
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