Anti-Fyn antibody
| Name | Anti-Fyn antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab13955 |
| Prices | $359.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Rat, Human, Mouse |
| Antigen | Recombinant protein expressed in E |
| Description | Rabbit Polyclonal |
| Gene | FYN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab13955 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13955, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Ab13955 staining human normal cerebellum tissue. Staining is localized to cytoplasm.Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Product References
Gene expression profiling identifies FYN as an important molecule in tamoxifen - Gene expression profiling identifies FYN as an important molecule in tamoxifen
Elias D, Vever H, Laenkholm AV, Gjerstorff MF, Yde CW, Lykkesfeldt AE, Ditzel HJ. Oncogene. 2015 Apr 9;34(15):1919-27.
Role of LRAT on the retinoid isomerase activity and membrane association of - Role of LRAT on the retinoid isomerase activity and membrane association of
Jin M, Yuan Q, Li S, Travis GH. J Biol Chem. 2007 Jul 20;282(29):20915-24. Epub 2007 May 15.