Anti-FZR1 antibody
| Name | Anti-FZR1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab89535 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | ICC/IF ICC/IF WB ELISA |
| Species Reactivities | Human |
| Antigen | Recombinant fragment: MDQDYERRLL RQIVIQNENT MPRVTEMRRT LTPASSPVSS PSKHGDRFIP SRAGANWSVN FHRINENEKS PSQNRKAKDA TSDNGKDGLA YSALLKNELL G, corresponding to amino acids 1-102 of human FZR1 (NP_057347 |
| Description | Mouse Monoclonal |
| Gene | FZR1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab89535 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab89535 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in methanol fixed (100%, 5min) MCF-7 cells at 10ug/ml.
Anti-FZR1 antibody (ab89535) at 5 µg/ml + immunogen at 0.2 µg
Anti-FZR1 antibody (ab89535) at 5 µg/ml + HeLa cell lysate at 50 µg