FZR1

Gene Symbol FZR1
Entrez Gene 51343
Alt Symbol CDC20C, CDH1, FZR, FZR2, HCDH, HCDH1
Species Human
Gene Type protein-coding
Description fizzy/cell division cycle 20 related 1 (Drosophila)
Other Description CDC20-like 1b|CDC20-like protein 1|cdh1/Hct1 homolog|fizzy-related protein homolog
Swissprots Q9UI96 Q9ULH8 Q9UM11 Q9UM10 Q86U66 Q9Y2T8 O75869 Q96NW8 Q9UNQ1
Accessions AAC62835 AAC62836 EAW69313 EAW69314 EAW69315 Q9UM11 AB013462 BAA86954 AB013463 BAA86955 AB033068 BAA86556 AF080397 AAF20266 AF083810 AAD52030 AF102507 AAD26623 AF102508 AAD26624 AF433157 AAL28117 BC013413 AAH13413 BC111760 BT007115 AAP35779 DB462260 DQ890739 ABM81665 HQ448330 ADQ32810 XM_005259573 XP_005259630 XM_011528053 XP_011526355 XR_936186 NM_001136197 NP_001129669 NM_001136198 NP_001129670 NM_016263 NP_057347
Function Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. {ECO:0000269|PubMed:18662541, ECO:0000269|PubMed:21596315, ECO:0000269|PubMed:9734353}.
Subcellular Location Isoform 2: Nucleus.
Tissue Specificity Isoform 2 is expressed at high levels in heart, liver, spleen and some cancer cell lines whereas isoform 3 is expressed only at low levels in these tissues. {ECO:0000269|PubMed:12797865}.
Top Pathways Cell cycle, Ubiquitin mediated proteolysis, Progesterone-mediated oocyte maturation

fzr (N-15) - sc-19398 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
fzr (N-15): sc-19398. Western blot analysis of fzr expression in HeLa (A) and SK-N-MC (B) whole cell lysates.

fzr (K-15) - sc-19399 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

fzr (AR38.2) - sc-53291 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
fzr (AR38.2): sc-53291. Western blot analysis of fzr expression in HeLa (A) Jurkat (B) and C2C12 (C) whole cell lysates.

fzr (B-6) - sc-166714 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
fzr (B-6): sc-166714. Western blot analysis of fzr expression in non-transfected 293T: sc-117752 (A), mouse fzr transfected 293T: sc-120348 (B) and HeLa (C) whole cell lysates.

fzr (DCS-266) - sc-56312 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
fzr (DCS-266): sc-56312. Western blot analysis of fzr expression in HeLa (A) and SK-N-MC (B) whole cell lysates.

fzr (SPM381) - sc-56381 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

Anti-FZR1 antibody - ab89535 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
ICC/IF image of ab89535 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab89535 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in methanol fixed (100%, 5min) MCF-7 cells at 10ug/ml.

Anti-FZR1 antibody - ab193549 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-FZR1 antibody [4C4] - ab124328 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 4C4
ab124328 at 5 ug/ml staining FZR1 in formalin fixed paraffin embedded Human spleen tissue using immunohistochemistry.

Anti-FZR1 antibody [AR38.2] - ab77885 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone AR38.2
All lanes : Anti-FZR1 antibody [AR38.2] (ab77885) at 2.5 µg/mlLane 1 : HeLa cell lysateLane 2 : 293 cell lysateLane 3 : Murine myeloid cells

Anti-FZR1 antibody [DH01 (DCS-266)] - ab3242 from Abcam

Prices $395.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone DH01 (DCS-266)
Overlay histogram showing HeLA cells stained with ab3242 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3242, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-FZR1 antibody - N-terminal - ab138832 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-FZR1 antibody - N-terminal (ab138832) at 1/100 dilution + Human placenta tissue lysate at 35 µg

Anti-FZR1 Antibody (Internal) - LS-C209950 from LifeSpan Bioscience

Prices $295.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal

Anti-FZR1 Antibody (aa53-82, AP) - LS-C235483 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-FZR1 Antibody (aa35-84) - LS-C116417 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
FZR1 antibody LS-C116417 Western blot of 721_B cell lysate.

Anti-FZR1 Antibody (aa53-82, APC) - LS-C235484 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-FZR1 Antibody (N-Terminus) - LS-C135252 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
FZR1 antibody LS-C135252 Western blot of Mouse Heart lysate.

Anti-FZR1 Antibody (aa53-82, Biotin) - LS-C235485 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-FZR1 Antibody (aa53-82, FITC) - LS-C235486 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-FZR1 Antibody (aa53-82, PE) - LS-C235491 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-FZR1 Antibody (aa53-82, Azide-free, HRP) - LS-C249029 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-FZR1 Antibody (aa53-81) - LS-C159693 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
FZR1 Antibody western blot of human placenta tissue lysates (35 ug/lane). The FZR1 antibody detected the FZR1 protein (arrow).

Anti-FZR1 Antibody (aa53-82) - LS-C329558 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-FZR1 Antibody - LS-C341807 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-FZR1 Antibody (N-Terminus) - LS-C345797 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of FZR Antibody with human Ovary Tumor lysate.
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