All lanes : Anti-GAB2 (phospho S623) antibody (ab92578) at 1/500 dilutionLane 1 : Extracts from HepG2 cells treated with PMA (125ng/ml, 30mins) Lane 2 : Extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins) with immunizing phosphopeptide at 10 µgLysates/proteins at 10 µg per lane.
Left panel: ab92578, at a 1/50 dilution, staining phosphorylated Gab2 in formalin-fixed, paraffin-embedded Human breast carcinoma tissue. Right panel: same, after pre-incubation with the immunizing phosphopeptide.