Anti-GAD67 antibody [K-87]
| Name | Anti-GAD67 antibody [K-87] |
|---|---|
| Supplier | Abcam |
| Catalog | ab26116 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | K-87 |
| Applications | ICC/IF ICC/IF FC IHC-P WB IHC-F |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide: RFRRTETDFSNLFARDLLPA , corresponding to amino acids 87-106 of Human GAD67 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | GAD1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-GAD67 antibody [K-87] (ab26116) at 1/2000 dilution + mouse cerebellum at 0.75 µgSecondaryHRP horse anti-mouse IgG at 1/5000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/22023_ab26116-244254-26116-WB.jpg)
Anti-GAD67 antibody [K-87] (ab26116) at 1/2000 dilution + mouse cerebellum at 0.75 µgSecondaryHRP horse anti-mouse IgG at 1/5000 dilution
ab26116, at a 1/2000 dilution, staining of fixed mouse cerebellum sections that underwent an antigen retrieval step.
Immunohistochemical staining of mouse pancreatic islet beta cells after antigen retrieval with ab26116 at a dilution of 1/1000.
ab26116 staining GAD67 in Rat brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X in PBS for 30 minutes and blocked with 10% goat serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/2000 in TBS + 1% BSA) for 18 hours at 4°C. A biotin-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.See Abreview
ICC/IF image of ab26116 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26116, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing PC12 cells stained with ab26116 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26116, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/22028_GAD67-Primary-antibodies-ab26116-3.jpg)
Overlay histogram showing PC12 cells stained with ab26116 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26116, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Product References
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