ab55424 staining gamma Synclein in Rat retina cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized by freezing and blocked with 2% serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/100 in 2% Triton + 2% Serum in PBS) for 8 hours at 4°C. An Alexa Fluor® 594-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.See Abreview
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab55424 (1/50 dilution), in the presence or absence of the immunizing peptide.Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab55424 (1/50 dilution), in the presence or absence of the immunizing peptide.
All lanes : Anti-gamma Synuclein antibody (ab55424) at 1/500 dilutionLane 1 : HT29 cell extract, without immunizing peptideLane 2 : HT29 cell extract, with immunizing peptide
ab55424 staining gamma synuclein in Mouse retinal tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in P) for 12 hours at 4°C. An Alexa Fluor®594-conjugated Donkey anti-rabbit polyclonal (1/1000) was used as the secondary antibody.See Abreview
ab55424 staining gamma Synuclein in murine retinal tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in formaldehyde, blocked using 10% serum for 30 minutes at 24°C, then incubated with ab55424 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.See Abreview
ab55424 staining gamma Synuclein in rat retinal tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in paraformaldehyde, blocked using 10% serum for 30 minutes at 24°C, then incubated with ab55424 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.See Abreview