Anti-gamma Synuclein antibody

• Supplier: Abcam
• Catalog: ab6169
• Prices: $390.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IP IHC-P IHC-F WB
• Species Reactivities: Rat, Human
• Antigen: Synthetic peptide EKEEVAEEAQSGGD corresponds to amino acids 114-127 of the human gamma synuclein protein
• Description: Rabbit Polyclonal
• Gene: SNCG
• Conjugate: Unconjugated

Product images

ab6169 was tested on recombinant protein only, 100ng recombinant protein each lane. 1= alpha-synuclein, 2 =  beta-synuclein, 3 = gamma-synuclein. The primary was 1/2000, secondary was alkaline phosphatase conjugated anti-rabbit from Sigma. We did not try it on crude samples such as brain or other tissue homogenates.

ICC/IF image of ab6169 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6169, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

IHC image of ab6169 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6169, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

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Newman AJ, Selkoe D, Dettmer U. PLoS One. 2013 Nov 20;8(11):e81314.

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Elevated expression of CYP1A1 and gamma-SYNUCLEIN in human ectopic (ovarian) - Elevated expression of CYP1A1 and gamma-SYNUCLEIN in human ectopic (ovarian)

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Effect of gamma-synuclein silencing on apoptotic pathways in retinal ganglion - Effect of gamma-synuclein silencing on apoptotic pathways in retinal ganglion

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Gamma-synuclein as a marker of retinal ganglion cells. - Gamma-synuclein as a marker of retinal ganglion cells.

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A candidate molecular signature associated with tamoxifen failure in primary - A candidate molecular signature associated with tamoxifen failure in primary

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Interaction of myocilin with gamma-synuclein affects its secretion and - Interaction of myocilin with gamma-synuclein affects its secretion and

Surgucheva I, Park BC, Yue BY, Tomarev S, Surguchov A. Cell Mol Neurobiol. 2005 Sep;25(6):1009-33.