![Anti-gamma Synuclein antibody [1H10D2] (ab47966) at 1/500 dilution + Truncated gamma Synuclein recombinant protein](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/22573_ab47966wbb.jpg)
Anti-gamma Synuclein antibody [1H10D2] (ab47966) at 1/500 dilution + Truncated gamma Synuclein recombinant protein
This image shows cytoplasmic staining of Human ovarian carcinoma tissue treated with ab47966 at 1/500 dilution.
This image shows cytoplasmic and nuclear staining of Human breast carcinoma tissue treated with ab47966 at 1/500 dilution.
![Overlay histogram showing SH-SY5Y cells stained with ab47966 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab47966, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/22576_gamma-Synuclein-Primary-antibodies-ab47966-1.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab47966 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab47966, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.