Anti-gamma Tubulin antibody
| Name | Anti-gamma Tubulin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab11321 |
| Prices | $394.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P IHC-F ICC/IF ICC/IF IHC WB |
| Species Reactivities | Mouse, Rat, Cat, Human, Pig, Xenopus, Zebrafish, Dog, Sea Urchin |
| Antigen | Synthetic peptide: AATRPDYISWGTQEQ-CG , with N-terminally added cysteine-glycine, conjugated to KLH, corresponding to amino acids 437-451 of Xenopus laevis gamma Tubulin |
| Description | Rabbit Polyclonal |
| Gene | TUBG1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab11321 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11321, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.
ab11321 at a 1/200 dilution staining gamma Tubulin in Xenopus neuroepithelial cells by Immunohistochemistry. (A) High-magnification projection of a stack of optical sections showing elongating neural epithelial cells stained for ?-tubulin (white lines indicate cell outline). (a') a-tubulin staining for cells shown in A. (B) Schematic of the closing neural tube indicating location of section shown in A (red box). (C) ?-tubulin staining in a projection of optical sections reveals centrosome-like foci at the apical surfaces of mature neuroepithelial cells. (c') Acetylated-a-tubulin staining reveals cell outlines in the ventral region of the closed Xenopus neural tube. (D) Schematic of the neural tube indicating location of section shown in C (red box). (E) Optical section showing a-tubulin in an embryo unilaterally injected with Shroom3-MO (right side is injected, left side is control). (e') ?-tubulin staining for cells shown in E.
Product References
GEF-H1 functions in apical constriction and cell intercalations and is essential - GEF-H1 functions in apical constriction and cell intercalations and is essential
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Defect of mitotic vimentin phosphorylation causes microophthalmia and cataract - Defect of mitotic vimentin phosphorylation causes microophthalmia and cataract
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Multiple primary cilia modulate the fluid transcytosis in choroid plexus - Multiple primary cilia modulate the fluid transcytosis in choroid plexus
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CDC2/SPDY transiently associates with endoplasmic reticulum exit sites during - CDC2/SPDY transiently associates with endoplasmic reticulum exit sites during
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Microtubule-dependent distribution of mRNA in adult cardiocytes. - Microtubule-dependent distribution of mRNA in adult cardiocytes.
Scholz D, Baicu CF, Tuxworth WJ, Xu L, Kasiganesan H, Menick DR, Cooper G 4th. Am J Physiol Heart Circ Physiol. 2008 Mar;294(3):H1135-44. doi:
Dishevelled controls apical docking and planar polarization of basal bodies in - Dishevelled controls apical docking and planar polarization of basal bodies in
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Shroom family proteins regulate gamma-tubulin distribution and microtubule - Shroom family proteins regulate gamma-tubulin distribution and microtubule
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The C-MYB locus is involved in chromosomal translocation and genomic duplications - The C-MYB locus is involved in chromosomal translocation and genomic duplications
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