TUBG1

Gene Symbol TUBG1
Entrez Gene 403695
Alt Symbol TUBG
Species Dog
Gene Type protein-coding
Description tubulin, gamma 1
Other Description GCP-1|gamma tubulin|gamma-1-tubulin|gamma-tubulin complex component 1|tubulin gamma-1 chain
Swissprots Q9GKK5
Accessions Q9GKK5 AF212974 AAG43544 NM_001003105 NP_001003105
Function Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation (By similarity). {ECO:0000250}.
Subcellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250}.

Anti-gamma Tubulin antibody - ab11321 from Abcam

Prices $394.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab11321 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11321, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.

Anti-gamma Tubulin antibody - ab11320 from Abcam

Prices $392.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab11320 at 1/400 dilution staining human neuroblastoma cells by ICC/IF. The cells were fixed with paraformaldehyde and blocked with serum and then incubated with the primary antibody for 16 hours. ab6717, a Goat polyclonal to Rabbit IgG H&L (FITC), was used as the secondary.See Abreview

Anti-gamma Tubulin antibody - Centrosome Marker - ab16504 from Abcam

Prices $398.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-gamma Tubulin antibody - Centrosome Marker (ab16504) at 1 µg/mlLane 1 : HeLa Whole Cell LysateLane 2 : HeLa Nuclear Cell LysateLane 3 : A431 Whole Cell LysateLane 4 : HeLa Whole Cell Lysate with Human gamma Tubulin peptide (ab17097) at 1 µg/mlLane 5 : HeLa Nuclear Cell Lysate with Human gamma Tubulin peptide (ab17097) at 1 µg/mlLane 6 : A431 Whole Cell Lysate with Human gamma Tubulin peptide (ab17097) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryAlexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilutionPerformed under reducing conditions.

Anti-gamma Tubulin antibody - Centrosome Marker - ab84355 from Abcam

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
gamma Tubulin was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to gamma Tubulin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab84355.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 48kDa; gamma Tubulin
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