Anti-gamma Tubulin antibody - Centrosome Marker

• Supplier: Abcam
• Catalog: ab16504
• Prices: $398.00
• Sizes: 50 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IP IHC IHC-F WB
• Species Reactivities: Mouse, Human, Zebrafish, Rat, Dog, Xenopus
• Antigen: Does not react with alpha or beta tubulin
• Description: Rabbit Polyclonal
• Gene: TUBG1
• Conjugate: Unconjugated

Product images

All lanes : Anti-gamma Tubulin antibody - Centrosome Marker (ab16504) at 1 µg/mlLane 1 : HeLa Whole Cell LysateLane 2 : HeLa Nuclear Cell LysateLane 3 : A431 Whole Cell LysateLane 4 : HeLa Whole Cell Lysate with Human gamma Tubulin peptide (ab17097) at 1 µg/mlLane 5 : HeLa Nuclear Cell Lysate with Human gamma Tubulin peptide (ab17097) at 1 µg/mlLane 6 : A431 Whole Cell Lysate with Human gamma Tubulin peptide (ab17097) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryAlexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilutionPerformed under reducing conditions.

ICC/IF image of ab16504 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab16504, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

SK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.5% Triton X-100 and incubated for 1 hour with  ab16504 (1/300). ab16504 staining is localized to the centrosome (red). The cells were counterstained with DAPI (blue). 100x magnification. The cells were blocked with 5% fetal bovine serum.

SK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.5% Triton X-100 and incubated for 1 hour with ab16504. The antibody clearly labels the centrosome (red). The cells were counterstained with DAPI (blue). The cells were blocked in 5% BSA.

ICC/IF image of ab16504 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16504, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. The cause of the background staining is uncertain, although a cytokeratin exisits of a similar molecular weight and amino acid sequence to that of the immunogen used to raise the antibody.

All lanes : Anti-gamma Tubulin antibody - Centrosome Marker (ab16504) at 1 µg/mlLane 1 : MarkerLane 2 : Zebrafish brain homogenate at 20 µgLane 3 : Zebrafish liver homogenate at 20 µgLane 4 : HeLa (Human epithelial carcinoma cell line) whole cell lysate at 20 µgSecondaryGoat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

ab16504 staining gamma Tubulin in E8 murine embryos by IHC - Wholemount.Samples were incubated with ab16504 at a 1/150 dilution for 12 hours at 4°C in 0.1% Triton/PBS. ab6717 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) was used as the secondary at a 1/250 dilution.See Abreview

Product References

Regulation of mitotic cytoskeleton dynamics and cytokinesis by integrin-linked - Regulation of mitotic cytoskeleton dynamics and cytokinesis by integrin-linked

Sikkema WK, Strikwerda A, Sharma M, Assi K, Salh B, Cox ME, Mills J. PLoS One. 2014 Jun 9;9(6):e98838.

Arsenite stress down-regulates phosphorylation and 14-3-3 binding of leucine-rich - Arsenite stress down-regulates phosphorylation and 14-3-3 binding of leucine-rich

Mamais A, Chia R, Beilina A, Hauser DN, Hall C, Lewis PA, Cookson MR, Bandopadhyay R. J Biol Chem. 2014 Aug 1;289(31):21386-400.

Coordinate regulation of DNA methylation and H3K27me3 in mouse embryonic stem - Coordinate regulation of DNA methylation and H3K27me3 in mouse embryonic stem

Hagarman JA, Motley MP, Kristjansdottir K, Soloway PD. PLoS One. 2013;8(1):e53880.

AAV exploits subcellular stress associated with inflammation, endoplasmic - AAV exploits subcellular stress associated with inflammation, endoplasmic

Johnson JS, Gentzsch M, Zhang L, Ribeiro CM, Kantor B, Kafri T, Pickles RJ, Samulski RJ. PLoS Pathog. 2011 May;7(5):e1002053.

Cdk1 and BRCA1 target gamma-tubulin to microtubule domains. - Cdk1 and BRCA1 target gamma-tubulin to microtubule domains.

Hubert T, Vandekerckhove J, Gettemans J. Biochem Biophys Res Commun. 2011 Oct 14;414(1):240-5. doi:

The retinitis pigmentosa protein RP2 links pericentriolar vesicle transport - The retinitis pigmentosa protein RP2 links pericentriolar vesicle transport

Evans RJ, Schwarz N, Nagel-Wolfrum K, Wolfrum U, Hardcastle AJ, Cheetham ME. Hum Mol Genet. 2010 Apr 1;19(7):1358-67.

Bcr/Abl interferes with the Fanconi anemia/BRCA pathway: implications in the - Bcr/Abl interferes with the Fanconi anemia/BRCA pathway: implications in the

Valeri A, Alonso-Ferrero ME, Rio P, Pujol MR, Casado JA, Perez L, Jacome A, Agirre X, Calasanz MJ, Hanenberg H, Surralles J, Prosper F, Albella B, Bueren JA. PLoS One. 2010 Dec 28;5(12):e15525.

Analyzing primary cilia by multiphoton microscopy. - Analyzing primary cilia by multiphoton microscopy.

Farnum CE, Williams RM, Donnelly E. Methods Cell Biol. 2009;94:117-35.