Anti-GAP43 antibody [EP890Y]

• Supplier: Abcam
• Catalog: ab75810
• Prices: $403.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP890Y
• Applications: WB IP ICC/IF FC IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: A synthetic peptide corresponding to residues on the C-terminus of human GAP43
• Description: Rabbit Monoclonal
• Gene: GAP43
• Conjugate: Unconjugated

Product images

ICC/IF image of ab75810 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75810, 1/50 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG(H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GAP43 antibody [EP890Y] (ab75810) at 1/200000 dilution + SH-SY5Y cell lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemical analysis of GAP43 in paraffin-embedded human brain tissue using ab75810 at 1/500 dilution.

ab75810 staining GAP43 in Mouse ear tissue sections by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections).  The sections were formaldehyde fixed, subjected to heat mediated antigen retrieval at pH 6 and blocked for 10 minutes at 25C. The primary antibody was diluted 1/500 and incubated with the sample for 1 hour at 25°C. An HRP polymer anti-rabbit IgG system was used undiluted, as the secondary antibody.See Abreview

ab75810 staining GAP43 in rat DRG by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed using sodium citrate pH 6.0. Samples were then blocked using 5% serum for 10 minutes at 37°C and incubated with ab75810 at a 1/200 dilution for 1 hour at 37°C. The secondary used was bioitin conjugated goat anti-rabbit IgG used at a 1/200 dilution.See Abreview

Overlay histogram showing SH-SY5Y cells stained with ab75810 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75810, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Product References

Amyloid precursor protein is an autonomous growth cone adhesion molecule engaged - Amyloid precursor protein is an autonomous growth cone adhesion molecule engaged

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Roles of GSK3beta in odor habituation and spontaneous neural activity of the - Roles of GSK3beta in odor habituation and spontaneous neural activity of the

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The novel caspase-3 substrate Gap43 is involved in AMPA receptor endocytosis and - The novel caspase-3 substrate Gap43 is involved in AMPA receptor endocytosis and

Han MH, Jiao S, Jia JM, Chen Y, Chen CY, Gucek M, Markey SP, Li Z. Mol Cell Proteomics. 2013 Dec;12(12):3719-31.