GAP43

Gene Symbol GAP43
Entrez Gene 2596
Alt Symbol B-50, PP46
Species Human
Gene Type protein-coding
Description growth associated protein 43
Other Description axonal membrane protein GAP-43|calmodulin-binding protein P-57|growth-associated protein 43|nerve growth-related peptide GAP43|neural phosphoprotein B-50|neuromodulin|neuron growth-associated protein 43|protein F1
Swissprots A8K0Y4 P17677
Accessions AAB28649 AAV88094 CAA59050 EAW79594 P17677 AF279774 AK123311 AK289699 BAF82388 AK290100 BAF82789 AK313658 BAG36412 AM392759 CAL37637 AM393509 CAL38386 BC007936 AAH07936 BT019771 AAV38576 BT019810 AAV38613 BX648458 DA701390 DQ893244 ABM84170 DQ896574 ABM87573 M25667 AAA52516 NM_001130064 NP_001123536 NM_002045 NP_002036
Function This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. {ECO:0000269|PubMed:14978216, ECO:0000269|PubMed:21152083}.
Subcellular Location Cell membrane {ECO:0000269|PubMed:14978216}; Peripheral membrane protein {ECO:0000269|PubMed:14978216}; Cytoplasmic side {ECO:0000269|PubMed:14978216}. Cell projection, growth cone membrane {ECO:0000269|PubMed:14978216}; Peripheral membrane protein {ECO:0000269|PubMed:14978216}; Cytoplasmic side {ECO:0000269|PubMed:14978216}. Cell junction, synapse {ECO:0000269|PubMed:14978216}. Cell projection, filopodium membrane {ECO:0000269|PubMed:14978216}; Peripheral membrane protein {ECO:0000269|PubMed:14978216}. Note=Cytoplasmic surface of growth cone and synaptic plasma membranes.

GAP43 (D9C8) Rabbit mAb - 8945 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D9C8
Western blot analysis of extracts from mouse neonatal brain and rat fetal brain using GAP43 (D9C8) Rabbit mAb.

GAP43 Antibody - 5307 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from mouse and rat brain using GAP43 Antibody.

GAP-43 (7B10) - sc-33705 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GAP-43 (7B10): sc-33705. Western blot analysis of GAP-43 expression in rat brain (A) and mouse brain (B) tissue extracts.

GAP-43 (B-5) - sc-17790 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GAP-43 (B-5): sc-17790. Immunoperoxidase staining of formalin fixed, paraffin-embedded human malignant glyoma tissue showing membrane and cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

GAP-43 (C-19) - sc-7457 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GAP-43 (C-19): sc-7457. Immunofluorescence staining of methanol-fixed IMR-32 cells showing membrane localization.

GAP-43 (H-100) - sc-10786 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GAP-43 (H-100): sc-10786. Western blot analysis of GAP-43 expression in mouse brain tissue extract.

GAP-43 (N-19) - sc-7458 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GAP-43 (N-19): sc-7458. Western blot analysis of GAP-43 expression in mouse brain extract.

GAP-43 (31) - sc-135915 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
GAP-43 (31): sc-135915. Western blot analysis of GAP-43 expression in rat cerebrum tissue extract.

p-GAP-43 (Ser 41) - sc-17109 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-GAP-43 (Ser 41)-R: sc-17109-R. Western blot analysis of GAP-43 phosphorylation in mouse brain tissue extract.

p-GAP-43 (Ser 41) - sc-135697 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
p-GAP-43 (Ser 41): sc-135697. Western blot analysis of GAP-43 phosphorylation in untreated (A) and lambda protein phosphatase-treated (B) rat prefrontal cortex tissue extracts.

Anti-GAP43 (phospho S41) antibody - ab75828 from Abcam

Prices $378.00
Sizes 500 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-GAP43 (phospho S41) antibody (ab75828) at 50 µg/mlLane 2 : Anti-GAP43 (phospho S41) antibody (ab75828) (Preabsorbed with immunising peptide))Lane 1 : Rat brain lysateLane 2 : Rat brain lysate pre-absorbed with Immunizing peptide

Anti-GAP43 (phospho S41) antibody - ab32588 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GAP43 (phospho S41) antibody (ab32588) at 1/1000 dilutionLane 1 : Rat brain lysateLane 2 : Rat brain lysate with Ser41 phosphopeptideLysates/proteins at 10 µg per lane.Observed band size : 50 kDa (why is the actual band size different from the predicted?)

Anti-GAP43 (phospho S41) antibody - ab194929 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of methanol fixed Hela cells labeling GAP43 (phospho S41) with ab194929 at 1/100 dilution

Anti-GAP43 (phospho S41) antibody [EPR1854(2)] - ab167162 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1854(2)
All lanes : Anti-GAP43 (phospho S41) antibody [EPR1854(2)] (ab167162) at 1/1000 dilutionLane 1 : Untreated SH-SY5Y cell lysatesLane 2 : SH-SY5Y cell lysates treated with Okadaic Acid and Calyculin A.Lysates/proteins at 10 µg per lane.

Anti-GAP43 antibody - ab11136 from Abcam

Prices $378.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-GAP43 antibody - ab137910 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-GAP43 antibody (ab137910) at 1/1000 dilution + SH-SY-5Y cells lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-GAP43 antibody - ab131482 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GAP43 antibody (ab131482) at 1/500 dilutionLane 1 : Mouse brain tissue extract with blocking peptideLane 2 : Mouse brain tissue extract

Anti-GAP43 antibody - ab47510 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical staining (paraffin embedded) of human breast carcinoma tissue. Left panel; ab47510 (1/50-1/100). Right panel; ab47510 (1/50-1/100) with peptide.

Anti-GAP43 antibody - ab167031 from Abcam

Prices $376.00
Sizes 200 µl
Host Chicken
Clonality Polyclonal
Immunofluorescent analysis of dissociated cell culture from an e13 Mouse brain, labeling GAP43 with ab167031 at 1/500 dilution.

Anti-GAP43 antibody - ab89619 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-GAP43 antibody (ab89619) at 1 µg/ml + rat brain tissue lysate at 50 µg

Anti-GAP43 antibody [EP890Y] - ab75810 from Abcam

Prices $403.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP890Y
ICC/IF image of ab75810 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75810, 1/50 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG(H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GAP43 antibody [EP890Y] (Alexa Fluor® 488) - ab196324 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP890Y
ab196324 staining GAP43 in U87MG cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196324 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in U87MG cells fixed with 4% formaldehyde (10 min).

Anti-GAP43 antibody [EP890Y] (Alexa Fluor® 647) - ab196540 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP890Y
ab196540 staining GAP43 in U87MG cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196540 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in U87MG cells fixed with 4% formaldehyde (10 min).

Anti-GAP43 antibody [EP928Y] - ab75843 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP928Y
Anti-GAP43 antibody [EP928Y] (ab75843) at 1/100000 dilution + human brain lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-GAP43 antibody [EPR5031] - ab134075 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5031
All lanes : Anti-GAP43 antibody [EPR5031] (ab134075) at 1/1000 dilutionLane 1 : SH-SY5Y lysateLane 2 : Human brain lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti-rabbit at 1/2000 dilution
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