Anti-GAP43 antibody - Neuronal Marker
| Name | Anti-GAP43 antibody - Neuronal Marker |
|---|---|
| Supplier | Abcam |
| Catalog | ab16053 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB IHC-F |
| Species Reactivities | Mouse, Rat, Human, Chimpanzee |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Rat GAP43 |
| Blocking Peptide | Rat GAP43 peptide |
| Description | Rabbit Polyclonal |
| Gene | GAP43 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunofluorescent staining obtained with ab16053 Rabbit polyclonal to GAP43 in rat dorsal root ganglion, 2 weeks after a spinal nerve injury. The staining is localised in the cytoplasm of some lesioned neurons (arrow in B) and in many axons (arrow heads in B). Image B (X40 objective) is a higher magnification of A (X20). The staining disappeared completely when the antibody was pre-incubated with the immunising (blocking) peptide (ab16379). Protocol details: Rats were intracardially perfused with 4% paraformaldehyde, DRG tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. IHC free floating was performed on the fixed, frozen and cut (20µm sections) rat tissue. Primary antibody ab16053 was incubated overnight at toom temperature at 1/300. Secondary antibody Alexa fluor 488 was incubated at 1/1000 for 2 hours at room temperature.
All lanes : Anti-GAP43 antibody - Neuronal Marker (ab16053) at 1 µg/mlLane 1 : spinal cord chung rat 1Lane 2 : spinal cord chung rat 2Lane 3 : spinal cord chung rat 3Lysates/proteins at 20 µg per lane.SecondaryDako Goat polyclonal to Rabbit IgG (HRP) at 1/2000 dilutionPerformed under reducing conditions.
Anti-GAP43 antibody - Neuronal Marker (ab16053) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab16053 staining GAP43 in murine dorsal root ganglia by Immunocytochemistry/ Immunofluorescence.Cells were fixed with methanol, permeabilized using 0.1% saponin/PBS, blocked with 4% BSA for 30 minutes at 25°C and then incubated with ab16053 at a 1/500 dilution for 14 hours at 4°C. The secondary antibody used was a DyLight 488 conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.See Abreview
Product References
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