Anti-GATA4 antibody
| Name | Anti-GATA4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab84593 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF IHC-F WB |
| Species Reactivities | Mouse, Rat, Human, Monkey, Dog, Pig |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human GATA4 |
| Description | Rabbit Polyclonal |
| Gene | GATA4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab84593 staining GATA4 (green) in mouse embryo tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized in 1% Triton X-100 and blocked with 10% goat serum for 1 hour at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50 in 10% goat serum) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody. Red - Myh6/7, Blue - DAPI.See Abreview
All lanes : Anti-GATA4 antibody (ab84593) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-GATA4 antibody (ab84593) at 1 µg/mlLane 1 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 2 : Human Fetal Heart - Total Protein Tissue Lysate Lane 3 : Heart (Mouse) Tissue LysateLane 4 : Heart (Rat) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Immunofluorescence image of Gata4 (ab84593) on human embryonic stem cell derived cardiomyocytes. The cells were fixed in methanol and permeabilized in 0.1% Triton-X-100. In this image you can see a cardiomyocyte stained in red with a cardiac alpha actinin antibody and both the cardiomyocyte and a fibroblast stained in green with ab84593.See Abreview
Product References
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Kanatsu-Shinohara M, Onoyama I, Nakayama KI, Shinohara T. Proc Natl Acad Sci U S A. 2014 Jun 17;111(24):8826-31. doi:
Postnatal development of the testis in the rat: morphologic study and correlation - Postnatal development of the testis in the rat: morphologic study and correlation
Picut CA, Remick AK, de Rijk EP, Simons ML, Stump DG, Parker GA. Toxicol Pathol. 2015 Apr;43(3):326-42.
The glucocorticoid-induced leucine zipper (gilz/Tsc22d3-2) gene locus plays a - The glucocorticoid-induced leucine zipper (gilz/Tsc22d3-2) gene locus plays a
Suarez PE, Rodriguez EG, Soundararajan R, Merillat AM, Stehle JC, Rotman S, Roger T, Voirol MJ, Wang J, Gross O, Petrilli V, Nadra K, Wilson A, Beermann F, Pralong FP, Maillard M, Pearce D, Chrast R, Rossier BC, Hummler E. Mol Endocrinol. 2012 Jun;26(6):1000-13.