GATA4

Gene Symbol GATA4
Entrez Gene 2626
Alt Symbol ASD2, TACHD, TOF, VSD1
Species Human
Gene Type protein-coding
Description GATA binding protein 4
Other Description GATA-binding factor 4|transcription factor GATA-4
Swissprots Q5IFM8 P43694 Q3MJ45 B7ZKZ4 B7ZKX0
Accessions AAA70335 ABY49125 ABY84859 ABY84860 ABY84861 ABY84862 ABY84863 ABY84864 ABY84865 ACD13586 ACD13587 EAW65620 EAW65621 P43694 AF180736 AF318320 AAL55827 AI192760 AK097060 BAG53416 AY740706 AAW51922 BC033672 BC068079 BC101580 AAI01581 BC105108 AAI05109 BC143434 AAI43435 BC143479 AAI43480 BM560562 BM768332 BQ439274 D78260 BAA11334 DA900813 DR003768 FJ169610 ACI04537 HY015197 HY023604 L34357 AAA58496 XM_005272384 XP_005272441 XM_005272385 XP_005272442 XM_005272386 XP_005272443 XM_006716248 XP_006716311 XM_006716249 XP_006716312 XM_011543817 XP_011542119 XM_011543818 XP_011542120 NM_001308093 NP_001295022 NM_001308094 NP_001295023 NM_002052 NP_002043
Function Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development. Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions. Acts as a transcriptional activator of ANF in cooperation with NKX2-5. Promotes cardiac myocyte enlargement. Required during testicular development. {ECO:0000269|PubMed:20081228, ECO:0000269|PubMed:21220346, ECO:0000269|PubMed:24000169}.
Subcellular Location Nucleus {ECO:0000269|PubMed:21220346, ECO:0000269|PubMed:24000169}.
Top Pathways cGMP-PKG signaling pathway

GATA-4 Antibody - 14353 from Cell Signaling Technology

Prices $239.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using GATA-4 Antibody (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, HeLa cells are negative for GATA-4.

p-GATA-4 (H-4) - sc-377543 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of GATA-4 phosphorylation in untreated (A), induction cocktail (sc-362324) treated (B) and induction cocktail (sc-362324) and lambda protein phosphatase (sc-200312A) treated (C) HeLa whole cell lysates. Antibodies tested include p-GATA-4 (H-4): sc-377543 (A,B,C).

GATA-4 (C-20) - sc-1237 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GATA-4 (C-20): sc-1237. Western blot analysis of GATA-4 expression in ATL-16T whole cell lysate.

GATA-4 (G-4) - sc-25310 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GATA-4 (G-4): sc-25310. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

GATA-4 (H-112) - sc-9053 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GATA-4 (H-112): sc-9053. Immunofluorescence staining of methanol-fixed ATL-16T cells showing nuclear localization.

p-GATA-4 (Ser 262) - sc-32823 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p-GATA-4 (Ser 262): sc-32823. Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing nuclear staining of glandular and lymphoid cells.

Anti-GATA4 (phospho S105) antibody - ab5245 from Abcam

Prices $384.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-GATA4 (phospho S105) antibody - ab92585 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GATA4 (phospho S105) antibody (ab92585) at 1 µg/mlLane 1 : Liver (Mouse) Tissue Lysate Lane 2 : Liver (Mouse) Tissue Lysate with Immunizing peptide at 1 µg/mlLane 3 : Liver (Mouse) Tissue Lysate with Non-modified peptide at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-GATA4 (phospho S105) antibody - ab63398 from Abcam

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of HepG2 cells using ab63398 at a 1:500.Left image untreated.Right image treated with phospho peptide.

Anti-GATA4 (phospho S262) antibody - ab195744 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GATA4 (phospho S262) antibody (ab195744) at 1/500 dilutionLane 1 : 293 whole cell lysates with antigen-specific peptideLane 2 : 293 whole cell lysatesLysates/proteins at 12.5 µg per lane.

Anti-GATA4 (phospho S262) antibody - ab61053 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
1/500-1/1000 ab61053 staining phosphorilated GATA4 on HuvEc cells in the absence (left) or presence (right) of the immunizing phosphopeptide.

Anti-GATA4 antibody - ab84593 from Abcam

Prices $398.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab84593 staining GATA4 (green) in mouse embryo tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized in 1% Triton X-100 and blocked with 10% goat serum for 1 hour at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50 in 10% goat serum) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody. Red - Myh6/7, Blue - DAPI.See Abreview

Anti-GATA4 antibody - ab61767 from Abcam

Prices $398.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GATA4 antibody (ab61767) at 1/500 dilutionLane 1 : HeLa cell extracts with no immunising peptideLane 2 : HeLa cell extracts with immunising peptide at 5 µgLysates/proteins at 5 µg per lane.

Anti-GATA4 antibody - ab61170 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
1/500-1/1000 ab61170 staining GATA4 on HeLa cells, in the absence or presence of the immunizing peptide.

Anti-GATA4 antibody - ab124265 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach linitis plastica tissue labelling GATA4 with ab124265 at 1/1000 (1µg/ml). Detection: DAB.

Anti-GATA4 antibody [6H10] - ab86371 from Abcam

Prices $378.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6H10
Anti-GATA4 antibody [6H10] (ab86371) at 1/500 dilution + rat fetal heart tissue lysate

Anti-GATA4 antibody [EPR4768] - ab134057 from Abcam

Prices $394.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4768
Overlay histogram showing Caco-2 cells stained with ab134057 (red line) at 1/600 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.

Anti-GATA4 antibody [EPR4768] (Alexa Fluor® 488) - ab194071 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4768
Overlay histogram showing HeLa cells stained with ab194071 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab194071, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-GATA4 antibody [EPR4768] (Alexa Fluor® 647) - ab194072 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4768
ab194072 staining  in HeLa cells. The cells were fixed with 4% formaldehyde (10min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab194072 at a working dilution of 1 in 50 (shown in red) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an Alexa Fluor® 488 Goat anti-Mouse secondary (ab150117) at 2μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-GATA4 antibody [EPR4768] (HRP) - ab194073 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4768
Anti-GATA4 antibody [EPR4768] (HRP) (ab194073) at 1/5000 dilution + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µgdeveloped using the ECL techniquePerformed under reducing conditions.

Anti-GATA4 Antibody (aa298-328) - LS-C159711 from LifeSpan Bioscience

Prices $295.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
GATA4 Antibody (Ascites)western blot of 293 cell line lysates (35 ug/lane). The GATA4 antibody detected the GATA4 protein (arrow).

Anti-GATA4 Antibody (Internal, clone EPR4768) - LS-C152305 from LifeSpan Bioscience

Prices $495.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4768
Immunofluorescent staining of HepG2 cells using anti-GATA4 antibody.

Anti-GATA4 Antibody (phospho-Ser105) - LS-C198994 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence of HepG2 cells, using GATA4 (Phospho-Ser105) Antibody. The sample on the right was incubated with synthetic peptide.

Anti-GATA4 Antibody (C-Terminus) - LS-C210110 from LifeSpan Bioscience

Prices $295.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal

Anti-GATA4 Antibody (aa71-120) - LS-C117901 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence of HeLa cells, using GATA4 (Ab-105) Antibody. The picture on the right is treated with the synthesized peptide.
Search more