Anti-GATA4 antibody
| Name | Anti-GATA4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab61767 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA IHC-P ICC/IF ICC/IF |
| Species Reactivities | Rat, Human |
| Antigen | Synthetic unphosphorylates peptide derived from human GATA4 around the phosphorylation site of serine 105 (P-V-SP-P-R) |
| Description | Rabbit Polyclonal |
| Gene | GATA4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-GATA4 antibody (ab61767) at 1/500 dilutionLane 1 : HeLa cell extracts with no immunising peptideLane 2 : HeLa cell extracts with immunising peptide at 5 µgLysates/proteins at 5 µg per lane.
Immunofluorescence analysis of HeLa cells, using ab61767 at a dilution of 1/500 - 1/1000, with and without the immunising peptide.
ab61767 (2µg/ml) staining GATA4 in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is weak nuclear staining.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Product References
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