Anti-GATA4 antibody [EPR4768]

• Supplier: Abcam
• Catalog: ab134057
• Prices: $394.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR4768
• Applications: WB FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human GATA4 (N terminal)
• Description: Rabbit Monoclonal
• Gene: GATA4
• Conjugate: Unconjugated

Product images

Overlay histogram showing Caco-2 cells stained with ab134057 (red line) at 1/600 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.

ab134057 staining GATA4 in the PC-3 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/150).  An Alexa Fluor®555-conjugated Goat anti-rabbit IgG(1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.

Anti-GATA4 antibody [EPR4768] (ab134057) at 1/2000 dilution + Mouse spleen lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

Anti-GATA4 antibody [EPR4768] (ab134057) at 1/2000 dilution + HepG2 lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), HRP-conjugated

Overlay histogram showing HeLa cells stained with ab134057, unpurified (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134057, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-GATA4 antibody [EPR4768] (ab134057) at 1/1000 dilution (unpurified)Lane 1 : HepG2 cell lysateLane 2 : Caco-2 cell lysateLane 3 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Immunofluorescent analysis of HepG2 cells labelling GATA4 with ab134057, unpuified, at 1/100 dilution.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD