Bcl-11B (D6F1) XP ® Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 12120
• Prices: $109.00, $277.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D6F1
• Applications: WB IP ICC/IF ChIP
• Species Reactivities: Human, Mouse, Rat, Monkey
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu391 of human Bcl-11B protein.
• Description: Rabbit Monoclonal
• Gene: BCL11B

Product images

Western blot analysis of extracts from MOLT-4 and Jurkat cells using Bcl-11B (D6F1) XP ® Rabbit mAb.

Immunoprecipitation of Bcl-11B from MOLT-4 cell extracts, using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Bcl-11B (D6F1) XP ® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Bcl-11B (D6F1) XP ® Rabbit mAb.

Confocal immunofluorescent analysis of Jurkat (positive; left) and HeLa (negative; right) cells using Bcl-11B (D6F1) XP ® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 Jurkat cells and either 10 μl of Bcl-11B (D6F1) XP ® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human CDKN1B Promoter Primers #11951, human MDM2 promoter primers, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.