Anti-GC1q R antibody [60.11]
| Name | Anti-GC1q R antibody [60.11] |
|---|---|
| Supplier | Abcam |
| Catalog | ab24733 |
| Prices | $403.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 60.11 |
| Applications | B/N IHC-P WB IP ELISA FC ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Bacterial expressed recombinant full length protein GC1q R |
| Description | Mouse Monoclonal |
| Gene | C1QBP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-GC1q R antibody [60.11] (ab24733) at 5 µg/mlLane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lane 5 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/23350_GC1q-R-Primary-antibodies-ab24733-6.jpg)
All lanes : Anti-GC1q R antibody [60.11] (ab24733) at 5 µg/mlLane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lane 5 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![Lane 1 : Mw standardsLanes 2 - 3 : Anti-GC1q R antibody [60.11] (ab24733) at 1/2000 dilutionLane 1 : As aboveLane 2 : 293T whole cell lysate at 20 µgLane 3 : Hela whole cell lysate at 20 µgSecondaryLanes 2 - 3 : Alexa Fluor® 680 conjugated goat anti-mousedeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/23351_ab24733_1.jpg)
Lane 1 : Mw standardsLanes 2 - 3 : Anti-GC1q R antibody [60.11] (ab24733) at 1/2000 dilutionLane 1 : As aboveLane 2 : 293T whole cell lysate at 20 µgLane 3 : Hela whole cell lysate at 20 µgSecondaryLanes 2 - 3 : Alexa Fluor® 680 conjugated goat anti-mousedeveloped using the ECL techniquePerformed under reducing conditions.
ab24733 used in Flow Cytometry.U937 cells were cultured in RPMI 10% FCS, treated with PMA (5nM) and, 6-12 hours later, cells were harvested, spinned and resuspended to 200,000 cells in 100µl. ab24733 used at 10µg/ml for 30 minutes at 4°C. A phycoerythrin conjugated goat anti-mouse polyclonal was used as the secondary antibody at a 1/100 dilution.See Abreview
IHC image of ab24733 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24733, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ICC/IF image of ab24733 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab24733 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
p33 (gC1q receptor) prevents cell damage by blocking the cytolytic activity of - p33 (gC1q receptor) prevents cell damage by blocking the cytolytic activity of
Westman J, Hansen FC, Olin AI, Morgelin M, Schmidtchen A, Herwald H. J Immunol. 2013 Dec 1;191(11):5714-21.
The multicompartmental p32/gClqR as a new target for antibody-based tumor - The multicompartmental p32/gClqR as a new target for antibody-based tumor
Sanchez-Martin D, Cuesta AM, Fogal V, Ruoslahti E, Alvarez-Vallina L. J Biol Chem. 2011 Feb 18;286(7):5197-203.
Activation of the plasma kallikrein-kinin system on human lung epithelial cells. - Activation of the plasma kallikrein-kinin system on human lung epithelial cells.
Vergiliana JF, Asokananthan N, Stewart GA. Biol Chem. 2010 Sep;391(9):1067-77.
Evidence that the two C1q binding membrane proteins, gC1q-R and cC1q-R, associate - Evidence that the two C1q binding membrane proteins, gC1q-R and cC1q-R, associate
Ghebrehiwet B, Lu PD, Zhang W, Keilbaugh SA, Leigh LE, Eggleton P, Reid KB, Peerschke EI. J Immunol. 1997 Aug 1;159(3):1429-36.
Evidence that the two C1q binding membrane proteins, gC1q-R and cC1q-R, associate - Evidence that the two C1q binding membrane proteins, gC1q-R and cC1q-R, associate
Ghebrehiwet B, Lu PD, Zhang W, Keilbaugh SA, Leigh LE, Eggleton P, Reid KB, Peerschke EI. J Immunol. 1997 Aug 1;159(3):1429-36.