Anti-GC1q R antibody [60.11]

• Supplier: Abcam
• Catalog: ab106047
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 60.11
• Applications: ICC/IF ICC/IF WB IP ELISA FA FC IHC-P
• Species Reactivities: Rat, Human, Hamster
• Antigen: Recombinant fragment, corresponding to amino acids 74-282 of mature Human GC1q R
• Description: Mouse Monoclonal
• Gene: C1QBP
• Conjugate: Unconjugated

Product images

IHC image of GC1q R staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab106047, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

ICC/IF image of ab106047 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab106047 at 10 ug/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Mouse IgG (H+L) (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Role of the receptor for the globular domain of C1q protein in the pathogenesis - Role of the receptor for the globular domain of C1q protein in the pathogenesis

Sansonno D, Tucci FA, Ghebrehiwet B, Lauletta G, Peerschke EI, Conteduca V, Russi S, Gatti P, Sansonno L, Dammacco F. J Immunol. 2009 Nov 1;183(9):6013-20.

gC1qR/p33 blockade reduces Staphylococcus aureus colonization of target tissues - gC1qR/p33 blockade reduces Staphylococcus aureus colonization of target tissues

Peerschke EI, Bayer AS, Ghebrehiwet B, Xiong YQ. Infect Immun. 2006 Aug;74(8):4418-23.

Surface expression of complement receptor gC1q-R/p33 is increased on the plasma - Surface expression of complement receptor gC1q-R/p33 is increased on the plasma

Grace KS, Bronson RA, Ghebrehiwet B. Biol Reprod. 2002 Mar;66(3):823-9.

gC1q-R/p33, a member of a new class of multifunctional and multicompartmental - gC1q-R/p33, a member of a new class of multifunctional and multicompartmental

Ghebrehiwet B, Lim BL, Kumar R, Feng X, Peerschke EI. Immunol Rev. 2001 Apr;180:65-77.

Evidence that the two C1q binding membrane proteins, gC1q-R and cC1q-R, associate - Evidence that the two C1q binding membrane proteins, gC1q-R and cC1q-R, associate

Ghebrehiwet B, Lu PD, Zhang W, Keilbaugh SA, Leigh LE, Eggleton P, Reid KB, Peerschke EI. J Immunol. 1997 Aug 1;159(3):1429-36.