Anti-GCLM antibody
| Name | Anti-GCLM antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab55436 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | WB FC |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 1-275 of Human GCLM (Gamma Glutamylcysteine Synthetase regulatory subunit) |
| Description | Mouse Monoclonal |
| Gene | GCLM |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
GCLM (ab55436) at 1ug/lane + HeLa cell lysate at 25ug/lane.
![Overlay histogram showing HeLa cells stained with ab55436 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55436, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/23498_GCLM-Primary-antibodies-ab55436-1.jpg)
Overlay histogram showing HeLa cells stained with ab55436 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55436, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Product References
4-Hydroxy hexenal derived from docosahexaenoic acid protects endothelial cells - 4-Hydroxy hexenal derived from docosahexaenoic acid protects endothelial cells
Ishikado A, Morino K, Nishio Y, Nakagawa F, Mukose A, Sono Y, Yoshioka N, Kondo K, Sekine O, Yoshizaki T, Ugi S, Uzu T, Kawai H, Makino T, Okamura T, Yamamoto M, Kashiwagi A, Maegawa H. PLoS One. 2013 Jul 23;8(7):e69415.
Hypoxia and kinase activity regulate lung epithelial cell glutathione. - Hypoxia and kinase activity regulate lung epithelial cell glutathione.
Jackson RM, Gupta C. Exp Lung Res. 2010 Feb;36(1):45-56.