Overlay histogram showing HeLa cells stained with ab126704 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126704, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
ab126704, at 1/50 dilution, staining GCLM in Paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
![All lanes : Anti-GCLM antibody [EPR6667] (ab126704) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : A673 cell lysateLane 3 : PC12 cell lysateLane 4 : A431 cell lysateLane 5 : K562 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/23507_GCLM-Primary-antibodies-ab126704-3.jpg)
All lanes : Anti-GCLM antibody [EPR6667] (ab126704) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : A673 cell lysateLane 3 : PC12 cell lysateLane 4 : A431 cell lysateLane 5 : K562 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution