Anti-GCN2 antibody
| Name | Anti-GCN2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab70214 |
| Prices | $385.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IP |
| Species Reactivities | Human, Rabbit, Horse, Bovine, Dog, Chimpanzee, Monkey, Ape, Orangutan |
| Antigen | Synthetic peptide corresponding to a region between N terminal residues 1 and 50 of human GCN2 |
| Description | Rabbit Polyclonal |
| Gene | EIF2AK4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-GCN2 antibody (ab70214) at 0.1 µg/mlLane 1 : Whole cell lysate from HeLa cells at 50 µgLane 2 : Whole cell lysate from HeLa cells at 15 µgLane 3 : Whole cell lysate from HeLa cells at 5 µgLane 4 : Whole cell lysate from 293T cells at 50 µgLane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Detection of Human GCN2 by Immunoprecipitation in Whole cell lysate from HeLa cells using ab70214 at 3 µg/mg lysate for IP and at 0.1 µg/ml for subsequent WB detection.
ICC/IF image of ab70214 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70214, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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