EIF2AK4

Gene Symbol EIF2AK4
Entrez Gene 440275
Alt Symbol GCN2, PVOD2
Species Human
Gene Type protein-coding
Description eukaryotic translation initiation factor 2 alpha kinase 4
Other Description GCN2 eIF2alpha kinase|GCN2-like protein|eukaryotic translation initiation factor 2-alpha kinase 4|general control nonderepressible 2
Swissprots Q9NSQ3 Q6DC97 Q9P2K8 Q9H5K1 C9JEC4 Q9NSZ5 Q69YL7 Q9UJ56 Q96GN6
Accessions EAW92388 Q9P2K8 AB037759 BAA92576 AJ243428 CAB58360 AK027011 BAB15625 AK074361 AL137627 CAB70849 AL137676 AL157497 CAB75678 AL832907 CAH10626 BC009350 AAH09350 BC014915 BC019914 BC061591 BC078179 AAH78179 BC146319 AAI46320 BX649048 CN286138 XM_005254392 XP_005254449 XM_011521599 XP_011519901 XM_011521600 XP_011519902 NM_001013703 NP_001013725
Function Can phosphorylate the alpha subunit of EIF2 and may mediate translational control. {ECO:0000250}.
Tissue Specificity Widely expressed. Expressed in the lung in smooth muscle cells of the pulmonary vessel wall, interstitial tissue and macrophages. {ECO:0000269|PubMed:10504407, ECO:0000269|PubMed:24292273}.
Top Pathways Protein processing in endoplasmic reticulum, Hepatitis C, Influenza A, Measles, Herpes simplex infection

GCN2 Antibody - 3302 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from ME180 and HT1376 cells that were untreated, treated with UV light (50mJ/cm2, 30 minutes), or subjected to nocodazole block (50 ng/ml, 24hrs), using GCN2 Antibody.

Phospho-GCN2 (Thr898) Antibody - 3301 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from SV-T2 cells, untreated or UV-treated (50 mJ/cm2 for 30 minutes), using Phospho-GCN2 (Thr898) Antibody.

GCN2 (F-7) - sc-374609 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GCN2 (F-7): sc-374609. Immunoperoxidase staining of formalin fixed, paraffin-embedded human thyroid gland tissue showing cytoplasmic staining of glandular cells.

GCN2 (H-300) - sc-66902 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

GCN2 (C-13) - sc-46338 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GCN2 (C-13): sc-46338. Western blot analysis of GCN2 expression in NIH/3T3 whole cell lysate.

GCN2 (P-13) - sc-46341 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GCN2 (P-13): sc-46341. Western blot analysis of GCN2 expression in MES-SA/D x5 (A), IMR-32 (B) and HeLa (C) whole cell lysates.

Anti-GCN2 (phospho T667) antibody [EPR2319Y] - ab68427 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2319Y
All lanes : Anti-GCN2 (phospho T667) antibody [EPR2319Y] (ab68427) at 1/2000 dilutionLane 1 : HeLa cell lysate - untreatedLane 2 : HeLa cell lysate - treated with LPLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti-Rabbit antibody at 1/2000 dilution

Anti-GCN2 (phospho T899) antibody [EPR2320Y] - ab75836 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2320Y
All lanes : Anti-GCN2 (phospho T899) antibody [EPR2320Y] (ab75836) at 1/1000 dilution (purified)Lane 1 : HeLa cell lysate - untreatedLane 2 : HeLa cell lysate - treated with CalyculinLysates/proteins at 10 µg per lane.SecondaryPeroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Anti-GCN2 antibody - ab70214 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GCN2 antibody (ab70214) at 0.1 µg/mlLane 1 : Whole cell lysate from HeLa cells at 50 µgLane 2 : Whole cell lysate from HeLa cells at 15 µgLane 3 : Whole cell lysate from HeLa cells at 5 µgLane 4 : Whole cell lysate from 293T cells at 50 µgLane 5 : Whole cell lysate from NIH3T3 cells at 50 µg

Anti-GCN2 antibody - ab137543 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GCN2 antibody (ab137543) at 1/1000 dilutionLane 1 : H1299 whole cell lysateLane 2 : Molt-4 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-GCN2 antibody - ab117101 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ab117101 at 1/50 staining GCN2 in Formalin-fixed, Paraffin-embedded Human brain (cortex) tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-GCN2 antibody [EPR5970(2)] - ab134053 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5970(2)
Overlay histogram showing HeLa cells stained with ab134053 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134053, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-GCN2 Antibody (aa1-50) - LS-C65762 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GCN2 Antibody (aa22-139, clone 12K94) - LS-C182847 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 12K94

Anti-GCN2 Antibody (aa1477-1504, AP) - LS-C256553 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GCN2 Antibody (aa1477-1504, APC) - LS-C256554 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GCN2 Antibody (aa1477-1504, Biotin) - LS-C256555 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GCN2 Antibody (aa1477-1504, PE) - LS-C256558 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GCN2 Antibody (aa1477-1504, FITC) - LS-C256556 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GCN2 Antibody (phospho-Thr667, clone EPR2319Y) - LS-C105284 from LifeSpan Bioscience

Prices $450.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2319Y
Western blot analysis on HeLa cell lysates using anti-Phospho-GCN2 (pR2319) antibody (LS-C105284), 1:2000 dilution. Cells were either (A) untreated (B) treated with LP.

Anti-GCN2 Antibody (aa1477-1504, Azide-free, HRP) - LS-C256560 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GCN2 Antibody (C-Terminus, AP) - LS-C277234 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GCN2 Antibody (aa1-30) - LS-C98326 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Anti-GCN2 Antibody (N-Terminus, clone EPR5970(2)) IHC-plus™ - LS-B7798 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5970(2)
Anti-GCN2 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7798 dilution 1:50.

Anti-GCN2 Antibody (C-Terminus, Biotin) - LS-C277236 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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