Anti-GCN2 antibody [EPR5970(2)]

• Supplier: Abcam
• Catalog: ab134053
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR5970(2)
• Applications: WB IHC-P ICC/IF FC
• Species Reactivities: Human
• Antigen: Synthetic peptide corresponding to residues near the N-terminus of Human GCN2
• Description: Rabbit Monoclonal
• Gene: EIF2AK4
• Conjugate: Unconjugated

Product images

Overlay histogram showing HeLa cells stained with ab134053 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134053, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-GCN2 antibody [EPR5970(2)] (ab134053) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLane 3 : MOLT4 cell lysateLane 4 : A549 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling GCN2 with ab134053 at 1/100 dilution.