Anti-Gemin 2 antibody [2E17]
| Name | Anti-Gemin 2 antibody [2E17] |
|---|---|
| Supplier | Abcam |
| Catalog | ab6084 |
| Prices | $370.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 2E17 |
| Applications | FC ICC/IF ICC/IF ELISA WB IP |
| Species Reactivities | Mouse, Human, Xenopus |
| Antigen | His-Gemin 2 fusion protein (amino acids 12 - 280, starting at 2nd methionine) |
| Description | Mouse Monoclonal |
| Gene | GEMIN2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab6084 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6084, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/23886_Gemin-2-Primary-antibodies-ab6084-1.jpg)
Overlay histogram showing HeLa cells stained with ab6084 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6084, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
HeLa cells were transfected with an ICP0-expressing plasmid before IF assays to detect the distribution of CB- and gem-associated proteins. ab6084 used at a 1/200 dilution to detect Gemin 2 (green). Bar 5 µm.
Product References
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