GEMIN2

Gene Symbol GEMIN2
Entrez Gene 8487
Alt Symbol SIP1, SIP1-delta
Species Human
Gene Type protein-coding
Description gem (nuclear organelle) associated protein 2
Other Description SMN interacting protein 1-delta|component of gems 2|gem-associated protein 2|gemin-2|survival of motor neuron protein interacting protein 1
Swissprots O14893 B2R9W8 Q9NS78 Q9NS77 Q9NS79 Q2M3B3 Q9H4F5
Accessions CAC16117 CAC16171 EAW65825 EAW65826 EAW65827 EAW65828 EAW65829 EAW65830 O14893 AB037701 BAB03508 AB037702 BAB03509 AB037703 BAB03510 AF027150 AAB82297 AK313947 BAG36665 BC028095 BC104968 AAI04969 CN484054 XM_011537238 XP_011535540 NM_001009182 NP_001009182 NM_001009183 NP_001009183 NM_003616 NP_003607
Function The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. {ECO:0000269|PubMed:18984161, ECO:0000269|PubMed:9323129}.
Subcellular Location Nucleus, gem. Cytoplasm. Note=Localized in subnuclear structures next to coiled bodies, called gems, which are highly enriched in spliceosomal snRNPs. Also found in the cytoplasm.
Top Pathways RNA transport

Gemin2 (1G9) - sc-33703 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Gemin2 (1G9): sc-33703. Western blot analysis of Gemin2 expression in HeLa (A), K-562 (B), Hep G2 (C) and SK-BR-3 (D) whole cell lysates.

Gemin2 (3F8) - sc-32806 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Gemin2 (3F8): sc-32806. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

Gemin2 (C-20) - sc-7801 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Gemin2 (C-20): sc-7801. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing nuclear staining of Purkinje cells, cells in granular layer and cells in molecular layer.

Gemin2 (E-6) - sc-166160 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Gemin2 (E-6): sc-166160. Western blot analysis of Gemin2 expression in HeLa (A) and K-562 (B) whole cell lysates.

Gemin2 (E-7) - sc-166162 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Gemin2 (E-7): sc-166162. Western blot analysis of Gemin2 expression in HeLa (A) and K-562 (B) whole cell lysates.

Gemin2 (G-6) - sc-166161 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Gemin2 (G-6): sc-166161. Western blot analysis of Gemin2 expression in HeLa (A) and K-562 (B) whole cell lysates.

Gemin2 (A-9) - sc-166187 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Gemin2 (A-9): sc-166187. Western blot analysis of Gemin2 expression in HeLa (A) and K-562 (B) whole cell lysates.

Gemin2 (N-19) - sc-7802 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Gemin2 (H-100) - sc-50404 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Gemin2 (H-100): sc-50404. Western blot analysis of Gemin2 expression in 293T (A) and HeLa (B) whole cell lysates.

Gemin2 (4) - sc-135918 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Gemin2 (4): sc-135918. Western blot analysis of Gemin2 expression in K-562 whole cell lysate.

Anti-Gemin 2 antibody - ab183067 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Gemin 2 antibody (ab183067) at 1/400 dilutionLane 1 : K562 cell lysateLane 2 : HeLa cell lysateLysates/proteins at 40 µg per lane.SecondaryGoat anti Rabbit IgG - H&L (HRP) at 1/10000 dilutiondeveloped using the ECL technique

Anti-Gemin 2 antibody - ab117639 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Human Colon Nerve, Formalin-Fixed, Paraffin-Embedded tissue, stained with ab117639 at 5µg/ml.

Anti-Gemin 2 antibody - ab124511 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence of Gemin 2 in HeLa cells with ab124511 at 20 ug/mL.

Anti-Gemin 2 antibody - ab83908 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Gemin 2 antibody (ab83908) at 1 µg/ml (in 5% skim milk/ PBS buffer) + HeLa cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-Gemin 2 antibody - ab88821 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-Gemin 2 antibody (ab88821) at 1 µg/ml + HeLa cell lysate at 50 µg

Anti-Gemin 2 antibody [2E17] - ab6084 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2E17
Overlay histogram showing HeLa cells stained with ab6084 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6084, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Gemin 2 antibody [EPR10040(B)] - ab150383 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10040(B)
All lanes : Anti-Gemin 2 antibody [EPR10040(B)] (ab150383) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : K562 cell lysateLane 3 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labeled goat anti-rabbit at 1/2000 dilution

Anti-SIP1 Antibody (C-Terminus) IHC-plus™ - LS-B5290 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-SIP1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5290 concentration 5 ug/ml.

Anti-SIP1 Antibody - LS-C332352 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded human ovarian cancer tissue using GEMIN2 antibody.

Anti-SIP1 Antibody (clone 2E17) - LS-C82217 from LifeSpan Bioscience

Prices $450.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2E17
Immunocytochemistry/ Immunofluorescence - Anti-Gemin 2 antibody [2E17] Image from Morency E et al, J Cell Biol. 2007 Jun 4;177(5):757-68, Fig 4f. DOI 10.1083/jcb.200612107

Anti-SIP1 Antibody (C-Terminus) - LS-C101831 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
SIP1 antibody LS-C101831 Western blot of HeLa lysate.

Anti-SIP1 Antibody (aa35-84) - LS-C135096 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
SIP1 antibody LS-C135096 Western blot of Mouse Brain lysate. Antibody concentration 1 ug/ml.

Anti-SIP1 Antibody (aa246-525) - LS-C311696 from LifeSpan Bioscience

Prices $250.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-SIP1 antibody used in Western blot.

Anti-SIP1 Antibody - LS-C341050 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SIP1 Antibody - LS-C341535 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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