Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Gemin 4 with ab70750 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-Gemin 4 antibody (ab70750) at 1/5000 dilutionLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgdeveloped using the ECL technique
Detection of Gemin 4 by Western Blot of Immunprecipitate. ab70750 at 1µg/ml staining Gemin 4 in HeLa whole cell lysate immunoprecipitated using ab70750 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: chemiluminescence with exposure time of 30 seconds.