GEMIN4

Gene Symbol GEMIN4
Entrez Gene 50628
Alt Symbol HC56, HCAP1, HHRF-1, p97
Species Human
Gene Type protein-coding
Description gem (nuclear organelle) associated protein 4
Other Description HCC-associated protein 1|component of gems 4|gem-associated protein 4|gemin-4
Swissprots Q9UG32 Q9Y4Q2 P57678 Q9NZS7
Accessions AAG24546 EAW90653 EAW90654 P57678 AF173856 AAF35283 AF177341 AAG17985 AK302722 BAG63941 AL080150 CAB45743 AL080167 CAB45755 BC020062 AAH20062 BC032084 BC036024 DB455185 EU832469 ACE87641 XM_005256667 XP_005256724 XM_005256668 XP_005256725 XM_005256670 XP_005256727 XM_011523910 XP_011522212 XM_011523911 XP_011522213 XM_011523912 XP_011522214 XM_011523913 XP_011522215 NM_015721 NP_056536
Function The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. {ECO:0000269|PubMed:18984161}.
Subcellular Location Cytoplasm. Nucleus. Nucleus, nucleolus. Nucleus, gem. Note=Localized in subnuclear structures next to coiled bodies, called gems, which are highly enriched in spliceosomal snRNPs and in the nucleolus.
Top Pathways RNA transport

Gemin4 (A-1) - sc-166078 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Gemin4 (A-1): sc-166078. Western blot analysis of Gemin4 expression in SK-N-SH whole cell lysate.

Gemin4 (A-2) - sc-166016 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Gemin4 (A-2): sc-166016. Western blot analysis of Gemin4 expression in SK-N-SH whole cell lysate.

Gemin4 (E-8) - sc-365424 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Gemin4 (E-8): sc-365424. Western blot analysis of Gemin4 expression in Hep G2 whole cell lysate.

Gemin4 (C-18) - sc-21437 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Gemin4 (C-18): sc-21437. Western blot analysis of Gemin4 expression in Hep G2 (A), A549 (B), SK-N-SH (C) and PC-3 (D) whole cell lysates.

Gemin4 (H-300) - sc-33765 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Gemin4 (F-2) - sc-166418 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Gemin4 (F-2): sc-166418. Western blot analysis of Gemin4 expression in 293T whole cell lysate.

Gemin4 (A-11) - sc-166017 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Gemin4 (A-11): sc-166017. Western blot analysis of Gemin4 expression in SK-N-SH whole cell lysate.

Gemin4 (17D10) - sc-136199 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

Anti-Gemin 4 antibody - ab31581 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Gemin 4 antibody (ab31581) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.

Anti-Gemin 4 antibody - ab70750 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Gemin 4 with ab70750 at 1/200 (1µg/ml). Detection: DAB.

Anti-Gemin 4 antibody - ab92535 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab92535 at 1/250 dilution staining Gemin 4 in Human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.

Anti-Gemin 4 antibody - ab22173 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-Gemin 4 antibody (ab22173) at 1/1000 dilutionLane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ugLane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ugSecondaryRabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

Anti-Gemin 4 antibody - ab98139 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Gemin 4 antibody (ab98139) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-GEMIN4 Antibody (aa287-336) - LS-C107764 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
GEMIN4 antibody LS-C107764 Western blot of Jurkat lysate.

Anti-GEMIN4 Antibody (aa273-301) - LS-C157771 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
GEMIN4 Antibody western blot of A549 cell line lysates (35 ug/lane). The GEMIN4 antibody detected the GEMIN4 protein (arrow).

Anti-GEMIN4 Antibody (C-Terminus) - LS-C287712 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human Gemin4 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Gemin4 used at a dilution of 1:200 (1 Detection: DAB.

Anti-GEMIN4 Antibody (C-Terminus) - LS-C286699 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Mouse Gemin4 by Immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-Gemin4 used at a dilution of 1:250. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.

Anti-GEMIN4 Antibody (C-Terminus) - LS-C66458 from LifeSpan Bioscience

Prices $545.00
Sizes 20 µg
Host Rabbit
Clonality Polyclonal

Anti-GEMIN4 Antibody (aa273-301, AP) - LS-C257665 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GEMIN4 Antibody (aa273-301, APC) - LS-C257666 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GEMIN4 Antibody (aa273-301, Biotin) - LS-C257667 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GEMIN4 Antibody (aa273-301, FITC) - LS-C257668 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GEMIN4 Antibody (aa273-301, Azide-free, HRP) - LS-C257669 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GEMIN4 Antibody (aa273-301, PE) - LS-C257670 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GEMIN4 Antibody (aa959-1058) - LS-C307670 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
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