Anti-Geminin antibody
| Name | Anti-Geminin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab12147 |
| Prices | $400.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P |
| Species Reactivities | Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Geminin |
| Description | Rabbit Polyclonal |
| Gene | GMNN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Geminin antibody (ab12147) at 1/500 dilutionLane 1 : 293 lysateLane 2 : 293 lysate with Human Geminin peptide (ab12408) at 1 µg/mlLysates/proteins at 5 µg/ml per lane.developed using the ECL techniquePerformed under reducing conditions.
ab12147 (1/400) staining geminin in assynchronous HeLa cells. Cells were fixed in paraformaldehyde and counterstained with DAPI in order to highlight the nucleus. Secondary antibody: goat anti-rabbit IgG conjugated to Cy3. Please refer to abreview for further details.See Abreview
Image courtesy of Human Protein Atlas ab12147 staining in human testis, showing staining of the cells in the ductus seminiferus (in brown). Paraffin embedded testis tissue was incubated with ab12147 (1:175 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.ab12147 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org.
Lane 1 : Anti-Geminin antibody (ab12147) at 1 µg/mlLane 2 : Anti-6X His tag® antibody - ChIP Grade (ab9108) at 1 µg/mlLane 1 : Human Geminin full length protein (ab40570)Lane 2 : Human Geminin full length protein (ab40570)Lysates/proteins at 0.1 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
Anti-Geminin antibody (ab12147) at 1/500 dilution + Cell lysate prepared from human HeLa nuclear extract at 50 µgSecondaryHRP-conjugated goat poyclonal to rabbit IgG at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
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Maya-Mendoza A, Merchut-Maya JM, Bartkova J, Bartek J, Streuli CH, Jackson DA. Cell Death Dis. 2014 Jul 24;5:e1351.
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